The impact of Quercetin-Berberine compound and Clove extract on Ovalbumin-induced asthmatic mice

Document Type : Research Paper

Author

Department. Of Microbiology, College of Veterinary Medicine, University of Baghdad, Baghdad province, Iraq.

10.22103/jab.2025.25794.1755

Abstract

Objective
Asthma is a chronic inflammatory disorder of the airways described by hyperresponsiveness and constructional remodeling, carried mostly by immune dysregulation. Indigenous combinations as berberine, quercetin, and clove extractive contain well-documented anti-inflammatory and antioxidant attributes. This investigation targeted to survey the immunomodulatory and protective reactions of a quercetin–berberine compound and clove extractive in an ovalbumin (OVA)-induced murine pattern of asthma.
Materials and Methods
Thirty Swiss mice of both sexes were randomly assigned to three groups (n = 10 per group). The first group obtained an oral dose of quercetin–berberine compound (100 mg/mL, 0.1 mL) once a week for 8 weeks. The second group was given treatment with clove extractive applying the same regimen. The third group acted as a positive control and was managed 10% OVA (0.3 mL, intraperitoneally) on day one and anew after 8 weeks to incite asthma. At the termination of the treatment phase, blood and serum samples were gathered for quantification of white blood cells (WBCs), immunoglobulin E (IgE), and interleukin-4 (IL-4), applying ELISA. All experimental groups were thereafter treated with OVA (0.3 mL, intraperitoneally) before euthanasia at week nine. Histopathological examination of the lungs and trachea was then carried out.
Results
Treatment with either quercetin–berberine compound or clove extractive meaningfully decreased WBC counts, IL-4, and IgE levels in comparison to the positive control group (p ≤ 0.05). Histopathological analysis disclosed a marked depletion in airway inflammation, cellular infiltration, and tissue destruction in the treatment groups, denoting an attenuation of OVA-incited pulmonary pathology. Both treatments revealed equivalent protective reactions, with evidence of restored tissue integrity in comparison to untreated controls.
Conclusions
The results offer that the quercetin–berberine compound and clove extractive exert potent immunomodulatory activity, alleviating inflammatory responses and tissue destruction in OVA-incited asthma. These outcomes emboss the therapeutic potential of plant-derived combinations as adjunctive or alternative plans in the administration of airway inflammation and asthma. Further investigations are warranted to clarify underlying molecular mechanisms and assess their clinical relevance.

Keywords

References

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Agricultural Biotechnology Journal
Volume 17, Issue 3
August 2025
Pages 385-406

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