Response of some parameters of local orange seedling (Citrus Sinensis L.) budding on Sour Orange to organic waste addition and Zinc spraying

Document Type : Research Paper

Authors

1 Department of Soil and Water Techniques, Technical College of Al-Mussaib, Al-Furat Al-Awsat Technical University, Iraq.

2 Department of Plant Production Technologies, Technical College of Al-Mussaib, Al-Furat Al-Awsat Technical University, Iraq.

Abstract

Objective
The goal of this study was to assess the effects of organic matter application and zinc foliar spraying on the vegetative growth of local orange seedlings (Citrus sinensis L.) grafted onto sour orange rootstock. Improving seedling growth and nutritional status under nursery conditions was the main aim of this research.
Materials and Methods
Cattle manure was used as a source of organic matter and applied at four levels: 0, 0.25, 0.50, and 0.75 kg per seedling. Zinc was applied as a foliar spray at three concentrations (0, 20, and 40 mg Zn L⁻¹) using hydrated zinc sulfate as the zinc source. The experiment was arranged in a factorial completely randomized design with three replications. We recorded several vegetative growth parameters. These parameters were: leaf nutrient content, seedling height, seedling dry weight, stem diameter, number of leaves, chlorophyll content (SPAD value), and leaf area. analysis of variance (ANOVA) was used to analyze data. Least Significant Difference (LSD) test at the 5% probability level was used to perform mean comparisons.
Results
The results of our study showed that if we increase the level of organic matter, all measured vegetative growth parameters can be significantly improved. For our study, the highest value was obtained at 0.75 kg per seedling. The values of the parameters also reached the following values: leaf nitrogen content of 1.88%, seedling height of 98.87 cm, seedling dry weight of 116.6 g, stem diameter of 1.23 cm, chlorophyll content of 45.37 SPAD units, number of leaves of 95.27 leaves per seedling, and leaf area of 21.27 dm2 per seedling. Zinc foliar spraying at 40 mg L⁻¹ also caused a significant increase in all growth traits, including height (94.45 cm), stem diameter (1.20 cm), leaf number (91.9 leaves per seedling), leaf area (20.7 dm²), chlorophyll content (45.48 SPAD units), dry weight (117.3 g), and improved leaf mineral content. The combined application of 0.75 kg organic matter with 40 mg Zn L⁻¹ resulted in the highest values for all studied characteristics.
Conclusions
It can be concluded that the combined use of organic matter and zinc foliar application effectively enhances the vegetative growth and nutritional status of local orange seedlings. The treatment consisting of 0.75 kg organic matter per seedling combined with 40 mg Zn L⁻¹ was the most effective, and can be recommended to improve seedling quality in citrus nurseries and support better orchard establishment.

Keywords


Abd Hamidi, J. K., & Arak, R. R. (2024). Effect of adding organic and nano-zinc fertilizer on flower growth indicators, yield, its components and medically active compounds of safflower plants. IOP Conference Series: Earth and Environmental Science, 1371(5), Article 052025. https://doi.org/10.1088/1755-1315/1371/5/052025
Ajeel, M. R., & Al-Hakeim, M. S. (2024). The influence of biological and organic fertilization and boron spraying in some soil characteristics. IOP Conference Series: Earth and Environmental Science, 1371(8), Article 082022. https://doi.org/10.1088/1755-1315/1371/8/082022
Ajeel, M. R., Hamid, M. M. H., & Al-Shahbani, I. R. (2025). The impact of biofertilizers, organic fertilizers, and foliar application of boron on yield characteristics of maize (Zea mays L.). Basrah Journal of Agricultural Sciences, 38(1), 312–323. https://doi.org/10.37077/25200860.2024.38.1.24
Al-Bamarny, S. F., & Abdulrhman, A. S. (2018). Using foliar application of Fe and GA3 to improve growth of two olive cv. (Olea europaea) transplants. Science Journal of University of Zakho, 6(3), 89–93. https://doi.org/10.25271/sjuoz.2018.6.3.510
Al-Mamouri, R. R. A., Asl, B. A., & Hussien, M. H. (2023). Effect of the growth regulator gibberellins and nano iron spraying on the content of oils, phenolic compounds and flavonoids in the roots of fennel plant. IOP Conference Series: Earth and Environmental Science, 1158(6), Article 062038. https://doi.org/10.1088/1755-1315/1158/6/062038
Almekh, M. T. A. (2025). Effect of adding poultry waste and foliar fertilization on the ready and absorbable elements of apricot seedlings. Pakistan Journal of Agricultural Research, 38(2), 1–11. https://doi.org/10.17582/journal.pjar/2025/38.2.01.11
Al-Shahbani, I. R. O., & Almekh, M. T. A. (2022). Effect of adding bio-fertilizer and spraying nano- and chelated iron fertilizer on the nutrient content of orange seedling leaves. IOP Conference Series: Earth and Environmental Science, 1060(1), Article 012012. https://doi.org/10.1088/1755-1315/1060/1/012012
Assi, S. L., Almeek, M. T. A., & Al-Ameer, H. K. A. (2021). Effect of irrigation periods, adding organic fertilizers and spraying with potassium on the growth of mandarin seedlings using irrigation water affected by salinity. International Journal of Agricultural and Statistical Sciences, 17(Suppl. 1), 1825–1833. https://connectjournals.com/03899.2021.17.1825
Aswad, A. H., Hamid, M. M., Almeekh, M. T. A., & Ajeel, M. R. (2026). Phosphorus adsorption and release dynamics in calcareous soil as affected by humic and fulvic acids. Journal of Degraded and Mining Lands Management, 13(1), 9463–9472. https://doi.org/10.15243/jdmlm.2026.131.9463
Başar, H., & Gürel, S. (2016). The influence of Zn, Fe and B applications on leaf and fruit absorption of table olive “Gemlik” based on phonological stages. Scientia Horticulturae, 198, 336–343. https://doi.org/10.1016/j.scienta.2015.11.028
Broadley, M. R., White, P. J., Hammond, J. P., Zelko, I., & Lux, A. (2007). Zinc in plants. New Phytologist, 173(4), 677–702. https://doi.org/10.1111/j.1469-8137.2007.01996.x
Cakmak, I. (2008). Enrichment of cereal grains with zinc: Agronomic or genetic biofortification? Plant and Soil, 302(1–2), 1–17. https://doi.org/10.1007/s11104-007-9466-3
Canellas, L. P., & Olivares, F. L. (2014). Physiological responses to humic substances as plant growth promoter. Chemical and Biological Technologies in Agriculture, 1, Article 3. https://doi.org/10.1186/2196-5641-1-3
Debnath, S. C., & Jain, S. M. (Eds.). (n.d.). Propagation and conservation of horticultural plants: In vitro and in vivo [Topical collection]. Agronomy. https://www.mdpi.com/journal/agronomy/topical_collections/propagation_plants
Downton, W. J. S., Grant, W. J. R., & Loveys, B. R. (1987). Carbon dioxide enrichment increases yield of Valencia orange. Australian Journal of Plant Physiology, 14(4), 493–501. https://doi.org/10.1071/PP9870493
Fernández, V., & Eichert, T. (2009). Uptake of hydrophilic solutes through plant leaves: Current state of knowledge and perspectives of foliar fertilization. Critical Reviews in Plant Sciences, 28(1–2), 36–68. https://doi.org/10.1080/07352680902743069
Fikry, A. M., Radhi, K. S., Abourehab, M. A. S., Abou Sayed-Ahmed, T. A. M., Ibrahim, M. M., Mohsen, F. S., Abdou, N. A., Omar, A. A., Elesawi, I. E., & El-Saadony, M. T. (2022). Effect of inorganic and organic nitrogen sources and biofertilizer on Murcott mandarin fruit quality. Life, 12(12), Article 2120. https://doi.org/10.3390/life12122120
Franzluebbers, A. J. (2002). Soil organic matter stratification ratio as an indicator of soil quality. Soil & Tillage Research, 66(2), 95–106. https://doi.org/10.1016/S0167-1987(02)00018-1
Hasanuzzaman, M., Bhuyan, M. B., Raza, A., Hawrylak-Nowak, B., Matraszek-Gawron, R., Nahar, K., & Fujita, M. (2020). Selenium toxicity in plants and environment: Biogeochemistry and remediation possibilities. In Selenium in plants (pp. 71–96). Elsevier. https://doi.org/10.1016/B978-0-12-812763-1.00004-3
Haynes, R. J. (1980). A comparison of two modified Kjeldahl digestion techniques for multi-element plant analysis with conventional wet and dry ashing methods. Communications in Soil Science and Plant Analysis, 11(5), 459–467. https://doi.org/10.1080/00103628009367053
Hernández, L. E., Ruiz, J. M., Espinosa, F., Álvarez-Fernández, A., & Carvajal, M. (2024). Plant nutrition challenges for a sustainable agriculture of the future. Physiologia Plantarum, 176(6), Article e70018. https://doi.org/10.1111/ppl.70018
Hesse, P. R. (1971). A textbook of soil chemical analysis. John Murray.
Hilgeman, R. H., & Reuther, W. (1967). Evergreen tree fruits. In R. M. Hagan, H. R. Haise, & T. W. Edminster (Eds.), Irrigation of agricultural lands (Agronomy Monograph 11, pp. 664–679). American Society of Agronomy. https://doi.org/10.2134/agronmonogr11.c40
Hoque, M. N., Imran, S., Hannan, A., Paul, N. C., Mahamud, M. A., Chakrobortty, J., Sarker, P., Irin, I. J., Brestic, M., & Rahman, M. S. (2022). Organic amendments for mitigation of salinity stress in plants: A review. Life, 12(10), Article 1632. https://doi.org/10.3390/life12101632
Hussein, A. K., Abdel Amir, H. K., & Fadel, A. H. (2025). Effect of cultivation methods, adding organic matter and nano-fertilizers on the yield components of (Vicia faba L.). IOP Conference Series: Earth and Environmental Science, 1487(1), Article 012077. https://doi.org/10.1088/1755-1315/1487/1/012077
Jackson, M. L. (1958). Soil chemical analysis. Prentice-Hall.
John, M. K. (1970). Colorimetric determination of phosphorus in soil and plant materials with ascorbic acid. Soil Science, 109(4), 214–220. https://doi.org/10.1097/00010694-197004000-00002
Jones, J. B., Jr., & Case, V. W. (1990). Sampling, handling, and analyzing plant tissue samples. In R. L. Westerman (Ed.), Soil testing and plant analysis (3rd ed., pp. 389–427). Soil Science Society of America. https://doi.org/10.2136/sssabookser3.3ed.c15
Li, Q., Chen, Y., Zhu, J., Liu, L., Liu, J., Cheng, C., & Li, L. (2024). Effects of organic substitution on the yield and quality of apples and residual nitrate-N leaching in soil. Agronomy, 14(3), Article 415. https://doi.org/10.3390/agronomy14030415
Miri Nargesi, M., Sedaghathoor, S., & Hashemabadi, D. (2022). Effect of foliar application of amino acid, humic acid and fulvic acid on the oil content and quality of olive. Saudi Journal of Biological Sciences, 29(5), 3473–3481. https://doi.org/10.1016/j.sjbs.2022.02.034
Page, A. L. (Ed.). (1982). Methods of soil analysis: Part 2. Chemical and microbiological properties (2nd ed., Agronomy Monograph No. 9). American Society of Agronomy; Soil Science Society of America. https://doi.org/10.2134/agronmonogr9.2.2ed
SAS Institute Inc. (2023). The GLIMMIX procedure. In SAS/STAT® 15.3 user's guide. Cary, NC: Author.
Spayd, S. E., Stevens, R. G., Wample, R. L., & Evans, R. G. (1991). Nitrogen fertilization and factors influencing grapevine cold hardiness. In Proceedings of the International Symposium on Nitrogen in Grapes and Wine: Seattle, Washington, USA 18–19 June 1991 (pp. 120–125). American Society for Enology and Viticulture.
Swietlik, D., & Zhang, L. (1994). Critical zinc+2 activities for sour orange determined with chelator-buffered nutrient solutions. Journal of the American Society for Horticultural Science, 119(4), 693–701. https://doi.org/10.21273/JASHS.119.4.693
Tattini, M., Bertoni, P., Landi, A., & Traversi, M. L. (1991). Effect of humic acids on growth and biomass partitioning of container-grown olive plants. Acta Horticulturae, 294, 75–80. https://doi.org/10.17660/ActaHortic.1991.294.7
Visconti, F., Intrigliolo, D. S., & Mirás-Avalos, J. M. (2023). Effects of the annual nitrogen fertilization rate on vine performance and grape quality for winemaking: Insights from a meta-analysis. Australian Journal of Grape and Wine Research, 2023, Article 7989254. https://doi.org/10.1155/2023/7989254
Wen, M., Zhang, J., Zheng, Y., & Yi, S. (2021). Effects of combined potassium and organic fertilizer application on Newhall navel orange nutrient uptake, yield, and quality. Agronomy, 11(10), Article 1990. https://doi.org/10.3390/agronomy11101990