Evaluation of the Effect of Moringa Oleifera L. Medicinal Plant Extract and Zeatin on In vitro Culture of Phalaenopsis Orchid Plant

Document Type : Research Paper

Authors

1 M.S. Student, Department of Horticultural Science and Engineering, Faculty of Agriculture and Natural Resources, University of Hormozgan, Bandar Abbas, Iran.

2 Assistant Professor, Department of Horticultural Science and Engineering, Faculty of Agriculture and Natural Resources, University of Hormozgan, Bandar Abbas, Iran.

3 Department of Horticultural Sciences, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

10.22103/jab.2024.23849.1585

Abstract

Introduction: Phalaenopsis orchid is known as the most popular orchid species in the horticulture industry due to its large, colorful and durable flowers, as well as its high adaptability, and it has a high economic value in international flower markets. Plant tissue culture technology has been widely used for rapid and high-scale propagation of this species. Moringa leaf is rich in natural cytokine hormones (zeatin), ascorbic acid and minerals such as calcium, potassium and iron, which can support Phalaenopsis orchid in vitro culture. Therefore, the purpose of this research is to evaluate the effect of Moringa oleifera and zeatin extract on the in vitro growth of Phalaenopsis orchid.
Material and methods: In order to carry out this research, after preparing Moringa medicinal plant extracts, different concentrations of plant extract and zeatin hormone were prepared and will be applied on Phalaenopsis orchid cultivation medium. After measuring the measured traits (including number of leaves, number of roots, leaf length, root length, seedling height, percentage of live, infected and browned seedlings), data analysis was done.
Results: The results showed that leaves were formed on some flowering stems after about two months. The contamination percentage of explants reached zero by the fourth disinfection treatment (S4). In this sterilization method, the combination of benomyl fungicide and mercury chloride was used, and contamination was removed by using these substances. Also, the highest rate of regeneration was observed in the fourth disinfection method and the vertical orientation of the explant (S4D2). The addition of 150 mg/liter of moringa leaf extract and 1.5 mg/liter of zeatin increased the number of leaves. Moringa leaf extract significantly increased root number, leaf length, root length and fresh and dry weight of seedlings and antioxidant activity. Moringa treatment with different concentrations reduced the percentage of orchid seedlings contamination to zero. Also, the application of moringa prevented the browning of the seedling.
Conclusion: In general, the treatment of moringa leaf extract with a concentration of 150 mg/L is suggested as an alternative to zeatin hormone in tissue culture of orchids and other ornamental plants.
Keywords: Tissue culture technology, Moringa Oleifera, Phalaenopsis Orchid, Plant Extract, Zeatin

Keywords

References

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Agricultural Biotechnology Journal
Volume 16, Issue 4
January 2025
Pages 1-32

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