ارزیابی بیوانفورماتیک و خصوصیات زیر واحد بزرگ روبیسکو در برخی از ژنوتیپ‌های کاهو

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه پژوهشی زراعت و اصلاح نباتات، پژوهشکده کشاورزی، دانشگاه زابل ایمیل

2 گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه زابل

3 دانش‌آموخته کارشناسی ارشد، گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه زابل

چکیده

هدف: کاهو یکی از قدیمی‌ترین سبزی‌های دنیاست که منشأ آن در هندوستان و آسیای مرکزی است. در این تحقیق تنوع ژنتیکی 15 ژنوتیپ کاهو با استفاده از توالی ژن rbcl مورد بررسی قرار گرفت.
مواد و روش­ها: توالی 15 ژنوتیپ کاهو ابتدا با روش PCR جداسازی و سپس توالی یابی شد. کیفیت توالی‌ها با استفاده از نرم‌افزار Chromas 2.1.1 بررسی و سپس با روش ClustalW توسط نرم‌افزار MegAlign 5 هم‌ردیف سازی شده و دندروگرام روابط تبار­زایی و ماتریس تفاوت و تشابه توالی‌ها ترسیم گردیدند. همچنین برای مدل‌سازی و اعتبار سنجی پروتئین rbcl از SWISS-MODEL و QMEAN و ارزیابی ساختار و کیفیت شیمیایی پروتئین از نقشه­های راماچاندران و سرور Pro-SA استفاده شد.
نتایج: بررسی شبکه­های متقابل پروتئین- پروتئین با استفاده از نرم‌افزار STRING نشان داد اکثر پروتئین­های کد شده با ژن­های matK، psb-tranH با پروتئین rbcl اثر متقابل دارند. نتایج حاصل از تجزیه خوشه­ای، ماتریس تشابه و واریانس مولکولی و همچنین 1< dN/dS، همگی حاکی از وجود شباهت و حفاظت‌شدگی بالای توالی­ rbcl بین ژنوتیپ­های مورد مطالعه بود.
نتیجه‌گیری کلی: نتایج نشان داد که برای بررسی روابط فیلوژنتیکی در سطح گونه توالی rbcl چندان مفید نیست لذا می­توان گفت که یکی از علل عدم موفقیت این ناحیه در تفکیک گونه­ها، تغییرپذیری کم آن در بین گونه­ها است.

کلیدواژه‌ها


عنوان مقاله [English]

Bioinformatics Analysis and Characterization of RuBisCO's large subunit in some lettuce genotypes

نویسندگان [English]

  • Bahman Fazeli nasab 1
  • bratali fekheri 2
  • Zahra Jomeh ghasem abadi 3
1 *Research Department of Agronomy and Plant Breeding, Agricultural Research Institute, University of Zabol, Zabol, Iran,
2 Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol;
3 Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol
چکیده [English]

Objective
Lettuce is among one of the oldest vegetables in the world originally lodge in India and Central Asia. This study aims to evaluate the genetic diversity of fifteen lettuce genotypes by using the rbcl gene and protein sequences.
 
Material and Methods
Using sequencing method, the rbcl gene sequence of of fifteen lettuce were sequenced. Chromas 2.1.1 software, MegAlign ver5 software (using ClustalW approach) were applied for generating the similarity matrix, genetic distances as the same as building phylogram of the studied sequences. The modeling and validation of rbcl protein structure were performed through SWISS-MODEL and QMEAN, respectively. Further steps were progressed using Ramadan and Pro-SAP servers.
 
Results
Investigating protein-protein cross-links using STRING showed most of proteins encoded by matK, psb (A, D and C)-tranH have the interaction with rbcl protein. The results of cluster analysis, similarity matrix, molecular-variance and dn/ds was indicated that the sequence of rbcl gene and protein is very similar and conserve among all genotypes.
 
Conclusion
The results were indicated that the rbcl sequence is not useful to study the phylogenetic relationships inter-species. Therefore, this point is trustworthy to say that one of the reasons for failing the segregation all lettuce genotypes is the tiny diversity of rbcl gene.
 

کلیدواژه‌ها [English]

  • lettuce
  • dN/dS
  • Lactucin
  • genetic diversity
  • rbcl gene
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