ارزیابی میزان خواص آنتی اکسیدانی اسانس، عصاره ی آبی و نانوذرات فلزی بیوسنتز شده از گیاه رازیانه (F. vulgare) و اثر ضدسرطانی آن ها بر روی دو لاین سلولی سرطان پستان (Sum-159 و Hs-578T)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری بیوتکنولوژی، دانشگاه محقق اردبیلی، دانشکده کشاورزی و منابع طبیعی، گروه مهندسی تولید و ژنتیک گیاهی، اردبیل، ایران.

2 استاد، دانشگاه محقق اردبیلی، دانشکده کشاورزی و منابع طبیعی، گروه مهندسی تولید و ژنتیک گیاهی، اردبیل، ایران

3 استاد، گروه تولید و ژنتیک گیاهی، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل

4 استادیار، دانشگاه علوم پزشکی‌، واحد اردبیل، دانشکده داروسازی، گروه فارماکوگنوزی‌، اردبیل، ایران

5 دانشجوی دکتری بیوتکنولوژی، دانشگاه محقق اردبیلی، دانشکده کشاورزی و منابع طبیعی، گروه مهندسی تولید و ژنتیک گیاهی، اردبیل، ایران

6 مرکز تحقیقات ژنومیک و انکولوژی فایزر، دانشگاه گرانادا، دولت منطقه‌ای اندلس، 18016 گرانادا، اسپانیا

چکیده

سرطان سینه شایع‌ترین نوع سرطان در بین زنان سراسر جهان است. استفاده از روش جراحی، شیمی درمانی و پرتودرمانی به عنوان بهترین روش سنتی جهت درمان این بیماری شناخته شده است. در سال‌های اخیر با پیشرفت نانوتکنولوژی در علوم پزشکی روش‌های نوینی جهت درمان بیماری سرطان ابداع شده است. با توجه به خواص دارویی گیاه رازیانه به‌دلیل وجود ترکیبات باارزش همانند فلاونوئید جهت درمان بیماری سرطان، در این پژوهش، ابتدا ویژگی‌های مورفولوژیکی و ساختاری نانوذرات فلزی بیوسنتز شده از عصاره آبی گیاه رازیانه پرداخته‌شد و در ادامه خواص آنتی‌اکسیدانی و ضدسرطانی اسانس، عصاره‌ی آبی این گیاه و نانوذرات بیوسنتز شده از آن مورد بررسی قرار گرفت. در این پژوهش پس از بیوسنتز نانوذرات آهن، مس، روی و نقره از عصاره آبی گیاه رازیانه (F. vulgare) و بررسی خواص آن‌ها از نظر اندازه به کمک XRD و تأیید ساختار به کمک FTIR‌، میزان ترکیبات ثانویه، میزان فعالیت آنتی‌اکسیدانی و ضدسرطانی اسانس و عصاره آبی گیاه رازیانه و نانوذرات آهن، مس، روی و نقره بیوسنتز شده از آن بر روی دو لاین سلولی Sum-159 و Hs-578T مورد ارزیابی قرار گرفت.
طبق نتایج به دست آمده از DLS نانوذرات فلزی بیوسنتز شده، به ترتیب اندازه نانوذرات آهن، مس، روی و نقره برابر با 35، 32، 33 و 34 نانومتر بود. نتایج حاصل از FTIR نیز وجود نانوذرات فلزی مذکور را تأیید کردند. هم‌چنین طبق نتایج به دست آمده از HPLC و اسپکتوفتومتری وجود ترکیبات بیوشیمیایی نظیر فلاونوئید و آنتوسیانین و متابولیت‌هایی نظیر روتین، کوئرستین و کمپفرول در عصاره آبی گیاه رازیانه تأیید شدند. از نظر میزان فعالیت آنتی اکسیدانی نیز بین تیمارهای مختلف اختلاف معنی‌داری مشاهده شد هم چنین مشاهده شد که با افزایش غلظت تیمارها میزان فعالیت آنتی‌اکسیدانی آن ها نیز افزایش داشت و بیشترین مقدار فعالیت آنتی‌اکسیدانی هر تیمار مربوط به بالاترین غلظت هر تیمار بود. بیشترین میزان فعالیت آنتی‌اکسیدانی (41/87 درصد) مربوط به نانوذره مس در غلظت 500 میلی‌گرم در میلی لیتر بود. از نظر میزان IC50 تیمارهای ذکر شده نیز مشخص شد که لاین سلولی سلولی Sum-159 در مقایسه با لاین سلولی Hs-578T در مقابل غلظت‌های برابر تیمارهای مختلف مقاومت بالاتری داشت. طبق نتایج به دست آمده میزان خواص ضدسرطانی اسانس و عصاره آبی گیاه رازیانه و نانوذرات فلزی بیوسنتز شده از آن نشان داد که درصد سلول‌های نکروزه شده، درصد سلول‌های موجود در مرحله پیش آپوپتوزی و پس آپوپتوزی به طور معنی‌داری تحت تأثیر نوع تیمار مورد استفاده شده، نوع لاین سلولی و اثر متقابل آن‌ها قرار گرفت.

کلیدواژه‌ها

عنوان مقاله [English]

Evaluation of antioxidant properties of essential oil, aqueous extract and metal nanoparticles biosynthesized from F. vulgare and their anticancer effect on two breast cancer cell lines (Sum-159, Hs-578T)

نویسندگان [English]

  • Shima Bourang 1
  • Sodabeh Jahanbakhsh Godehkahriz 2
  • Rasool Asghari Zakaria 3
  • Hamed Parsa 4
  • Mehran Noruzpuor 5
  • Calahorra Jesús 6
1 Ph.D. Student, of Agricultural Biotechnology, Department of plant genetics and production engineering, University of Mohaghegh Ardabili, Ardabil, Iran.
2 Professor, Department of Plant Genetics and Production Engineering, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran.
3 Professor, Department of Plant Production and Genetics, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
4 Assistant Professor, Department of Pharmacognosy, Faculty of Pharmacy, Ardabil. Iran
5 Ph. D. Student, of Agricultural Biotechnology, Department of plant genetics and production engineering, University of Mohaghegh Ardabili, Ardabil, Iran.
6 GENYO, Centre for Genomics and Oncological Research, Pfizer University of Granada, Andalusian Regional Government, 18016 Granada, Spain
چکیده [English]

Objective
Breast cancer is still the most common cancer among women all over the world. Using surgery, chemotherapy, and radiation therapy is known as the best traditional method to treat this disease. In recent years, with the advancement of nanotechnology in medical science and treatment, new methods of cancer treatment have been investigated. Considering the medicinal and therapeutic properties of F.vulgare due to the presence of valuable compounds such as flavonoids for the treatment of cancer, in this research, first, the morphological and structural characteristics of metal nanoparticles biosynthesized from the aqueous extract of F.vulgare were discussed, then the antioxidant and anticancer properties of essential oil, aqueous extract of this plant and the biosynthesized nanoparticles were investigated.
Materials and methods
In this research, after the biosynthesis of iron, copper, zinc, and silver nanoparticles from the aqueous extract of fennel plant (F. vulgare) and their properties in terms of size with XRD and confirmation of the structure by FTIR, the amount of secondary metabolites, the amount of antioxidant and anticancer activity of the essential oil And the aqueous extract of fennel plant (F. vulgare) and iron, copper, zinc and silver nanoparticles biosynthesized from it were evaluated on the Sum-159 and the Hs-578T cell lines.
Results
According to the results obtained from DLS of biosynthesized metal nanoparticles, the sizes of iron, copper, zinc, and silver nanoparticles were 35, 32, 33, and 34 nm, respectively. The results of FTIR also confirmed the existence of the synthesized metal nanoparticles. Also, according to the results of HPLC and spectrophotometry, the presence of biochemical compounds such as flavonoid and anthocyanin and metabolites such as rutin, quercetin, and kaempferol in the aqueous extract of fennel plant was confirmed. In terms of antioxidant activity, a significant difference was observed between different treatments, it was also observed that with the increase in the concentration of the treatments, the amount of their antioxidant activity also increased, and the highest amount of antioxidant activity of each treatment was related to the highest concentration of each treatment. The highest amount of antioxidant activity (87.41%) was related to copper nanoparticles at a concentration of 500 mg/ml. In terms of the IC50 value of the mentioned treatments, it was also found that the Sum-159 cell line had higher resistance compared to the Hs-578T cell line against equal concentrations of different treatments.

کلیدواژه‌ها [English]

  • Aqueous extract
  • Breast cancer
  • F. vulgare
  • Hs-578T
  • and Sum-159

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مجله بیوتکنولوژی کشاورزی
دوره 16، شماره 1
فروردین 1403
صفحه 235-266

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