سنتز سبز نانوذرات اکسیدآهن، مس، اکسیدروی و نقره از عصاره آبی گیاه رازیانه (F. vulgare) و ارزیابی خواص ساختاری و ضدمیکروبی آن ها

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری بیوتکنولوژی، دانشگاه محقق اردبیلی، دانشکده کشاورزی و منابع طبیعی، گروه مهندسی تولید و ژنتیک گیاهی، اردبیل، ایران.

2 گروه مهندسی تولید و ژنتیک به نژادی ، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران

3 استاد، گروه تولید و ژنتیک گیاهی، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل

4 استادیار، دانشگاه علوم پزشکی‌، واحد اردبیل، دانشکده داروسازی، گروه فارماکوگنوزی‌، اردبیل، ایران

5 گروه تولید و ژنتیک گیاهی، دانشگاه محقق اردبیلی، اردبیل.

چکیده

هدف: رازیانه (Foeniculum vulgare) دارای ترکیبات دارویی مهم با توانایی مهار آسیب‌‌های اکسیداتیوی DNA‌‌ است. در پژوهش حاضر ابتدا نانوذرات فلزی از عصاره آبی اندام هوایی گیاه رازیانه بیوسنتز شده و پس از ارزیابی ساختار و اندازه نانوذرات، به بررسی تأثیر عصاره آبی حاصل از اندام هوایی گیاه رازیانه و نانوذرات فلزی بیوسنتز شده از آن بر روی رشد و زنده‌‌مانی باکتری‌‌های  E. coli و S. aureus پرداخته شد.
مواد و روش‌ها: اندازه نانوذرات حاصل به کمک میکروسکوپ الکترونی روبشی (‌‌SEM) و توزیع اندازه نانوذرات (DLS) و ساختار شیمیایی و پیوندهای موجود در ترکیب نانوذرات به کمک FTIR بررسی شدند. میزان فعالیت ضدباکتریایی عصاره حاصل (در غلظت 20، 10، 5 و 5/2 میکرولیتر در میلی‌‌لیتر) و نانوذرات اکسیدآهن (در غلظت 10، 5، 5/2 و 25/1 میکروگرم در میلی‌‌لیتر)، مس، اکسیدروی و نقره (در غلظت 2، 1، 5/0 و 25/0 میکروگرم در میلی‌‌لیتر) بر محیط کشت‌های حاوی باکتری E. coli و S. aureus به کمک روش صفحات انتشار و حداقل غلظت بازدارنده (MIC) مورد بررسی قرار گرفت.
نتایج: طبق نتایج حاصل از SEM نانوذرات اکسیدآهن، مس، نقره و اکسیدروی بیوسنتز شده دارای اندازه متوسط به ترتیب 25، 22، 23 و 24 نانومتر بود که می‌‌تواند از کارآیی بالایی در فرایند مقابله با باکتری‌‌های مختلف داشته باشد. طبق نتایج حاصل، باکتری E. coli نسبت به S. aureus به غلظت‌‌های برابر تیمارهای مورد استفاده حساس‌‌تر بوده و هاله‌‌های بزرگ‌‌تری را ایجاد کردند. هم‌‌چنین اندازه هاله‌‌های تشکیل شده نیز وابسته به غلظت بوده و با افزایش غلظت، اندازه آن‌‌ها افزایش یافت. درمیان تیمارهای استفاده شده، استفاده از نانوذرات نقره بیوسنتز شده (به خصوص در غلظت‌‌های 10، 5، 5/2 و 25/1 میلی‌‌گرم) خواص ضدباکتریایی بیشتری داشت. از نظر میزان زنده‌‌مانی و رشد باکتری‌‌های کشت شده به کمک روش MIC‌‌، نیز میزان IC50 باکتری E. coli نسبت به باکتری S. aureus در غلظت‌‌های برابر تیمارهای اعمال شده پایین‌‌تر بوده و بیانگر حساسیت بالای سویه باکتری E. coli نسبت به باکتری S. aureus بود.
نتیجه‌گیری: عصاره آبی گیاه رازیانه و نانوذرات فلزی بیوسنتز شده از آن از خواص بالایی در کنترل باکتری S. aureus به عنوان باکتری گرم مثبت و  E. coliبه عنوان باکتری گرم منفی برخوردار است. در این پژوهش مشخص شد که نانوذرات نقره بیوسنتز شده از عصاره آبی گیاه رازیانه در مقایسه با نانوذرات اکسیدآهن، اکسیدروی و مس از خواص ضدباکتریایی بالایی برخوردار است.

کلیدواژه‌ها

عنوان مقاله [English]

Green synthesis of iron oxide, copper, zinc oxide and silver nanoparticles from aqueous extract of F. vulgare and evaluation of their structural and antimicrobial properties

نویسندگان [English]

  • Shima Bourang 1
  • Sodabeh Jahanbakhsh Godehkahriz 2
  • Rasool Asghari Zakaria 3
  • Hamed Parsa 4
  • Mehran Noruzpuor 5
1 Ph.D. Student, of Agricultural Biotechnology, Department of plant genetics and production engineering, University of Mohaghegh Ardabili, Ardabil, Iran.
2 Department of plant genetics and production engineering, Faculty of agriculture and natural resources, University of Mohaghegh Ardabili, Ardabil, Iran
3 Professor, Department of Plant Production and Genetics, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
4 Assistant Professor, Department of Pharmacognosy, Faculty of Pharmacy, Ardabil. Iran
5 Department of Plant Production and Genetics, Mohaghegh Ardabili University, Ardabil.
چکیده [English]

Objective
Fennel (Foeniculum vulgare) has important medicinal compounds with the ability to inhibit DNA oxidative damage. In the present study, metal nanoparticles were first biosynthesized from the aqueous extract of the aerial parts of the fennel plant, and after evaluating the structure and size of the nanoparticles, the effect of the aqueous extract obtained from the aerial parts of the fennel plant and the metal nanoparticles biosynthesized from it was investigated on the growth and survival of bacteria. E. coli and S. aureus were investigated.
Materials and methods
The size of the resulting nanoparticles was analyzed using scanning electron microscope (SEM) and nanoparticle size distribution (DLS), and the chemical structure and bonds in the composition of nanoparticles were investigated using FTIR. The amount of antibacterial activity of the resulting extract (at a concentration of 20, 10, 5 and 2.5 µl/ml) and iron oxide nanoparticles (at a concentration of 10, 5, 2.5 and 1.25 µl/ml), copper, zinc oxide and silver (in concentrations of 2, 1, 0.5 and 0.25 µl/ml) on culture media containing E. coli and S. aureus bacteria were investigated using the diffusion plate method and the minimum inhibitory concentration (MIC).
Results
According to the SEM results, biosynthesized iron oxide, copper, silver and zinc oxide nanoparticles had an average size of 25, 22, 23 and 24 nm, respectively, which can be highly effective in the process of dealing with different bacteria. According to the results, E. coli bacteria were more sensitive than S. aureus to concentrations equal to the treatments used and created larger halos. Also, the size of the formed halos depended on the concentration and their size increased with the increase of the concentration. Among the treatments used, the use of biosynthesized silver nanoparticles (especially in concentrations of 10, 5, 2.5 and 1.25 mg) had more antibacterial properties. In terms of the survival rate and growth of bacteria cultured using the MIC method, the IC50 of E. coli bacteria is lower than that of S. aureus bacteria at concentrations equal to the applied treatments, indicating the high sensitivity of the bacterial strain. E. coli compared to S. aureus bacteria.
Conclusions
Aqueous extract of fennel plant and metal nanoparticles biosynthesized from it have high properties in controlling S. aureus bacteria as gram positive bacteria and E. coli as gram negative bacteria. In this research, it was found that silver nanoparticles biosynthesized from the aqueous extract of fennel plant have high antibacterial properties compared to iron oxide, zinc oxide and copper nanoparticles.

کلیدواژه‌ها [English]

  • antibacterial properties
  • F. vulgare
  • green synthesis
  • metal nanoparticles

مراجع

محمدآبادی محمدرضا، گلکار افروز، عسکری حصنی مجید (1402) اثر رازیانه (Foeniculum vulgare) بر بیان ژن فاکتور 1 رشد شبه انسولین در بافت شکمبه گوسفند کرمانی. مجله بیوتکنولوژی کشاورزی، 15(4)، 239-256.
جعفری احمدآبادی سید علی اصغر، عسکری‌‌همت حشمت‌‌اله، محمدآبادی محمدرضا (1402) تاثیر شاهدانه بر بیان ژن DLK1 در بافت‌ قلب بره‌های کرمانی. مجله بیوتکنولوژی کشاورزی، 15(1)، 217-234.
شکری سمیرا، خضری امین، محمدآبادی محمدرضا، خیرالدین حمید (1402) بررسی بیان ژنMYH7  در بافت‌های ران، دست و راسته بره‌های پرواری نژاد کرمانی. مجله بیوتکنولوژی کشاورزی، 15(2)، 217-236.125.
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مجله بیوتکنولوژی کشاورزی
دوره 16، شماره 3
مهر 1403
صفحه 60-88

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