فعالیت ضد باکتریایی نانوذرات نقره سنتز شده با عصاره هگزان برخی از جلبک‌های آب شیرین در برابر باکتری‌های مقاوم به چند دارو

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه زیست شناسی، دانشکده علوم، دانشگاه ثی قار، ثی قار، 64001، عراق

چکیده

هدف: باکتری‌های مقاوم به آنتی بیوتیک به دلیل استفاده ناخواسته از آنتی بیوتیک‌ها به یک نگرانی جهانی تبدیل شده‌اند که منجر به ایجاد سویه‌های باکتریایی مقاوم به بسیاری یا همه آنتی بیوتیک‌های موجود شده است. متابولیت‌های اولیه و ثانویه موجود در جلبک‌ها نقش عمده‌ای در تبدیل نیترات نقره به نانوذرات نقره (AgNPs) دارند.
مواد و روش‌ها: در فرآیند ساخت نانوذرات از عصاره هگزانی برخی از جلبک‌های آب شیرین استفاده شد. تغییر رنگ محلول واکنش از زرد به قهوه ای تیره به دلیل تحریک رزونانس پلاسمون سطحی به عنوان شاهدی برای این امر است. بر اساس تحقیقات انجام شده با استفاده از تبدیل فوریه مادون قرمز (FTIR) برای شناسایی گروه جلبک‌های موثر که در تشکیل آن‌ها نقش دارند، AgNPها با استفاده از طیف سنجی UV-Vis شناسایی شدند، پروتئین‌ها و فنل‌ها نقش مهمی در تشکیل نانوذرات نقره ایفا می‌کنند. نانوذرات یک میکروسکوپ الکترونی روبشی (SEM) برای مشخص کردن شکل‌ها و اندازه‌های نانوذرات نقره سنتز شده، که شامل ساختارهای کروی، میله‌ای و شش ضلعی است، استفاده شد. باکتری‌های مقاوم به چند دارو (MDR) تشخیص داده شده توسط سیستم Vitek Compact 2  برای آزمایش فعالیت ضد باکتریاییAgNP ها استفاده شد.
نتایج: مطالعه بر روی اثر ضد باکتریایی نانوذرات بیوسنتزی نقره در برابر جدایه‌های منتخب باکتری MDR انجام شد. نتایج نشان داد که نانوذرات نقره تهیه‌شده از عصاره هگزانی جلبک‌های جدا شده در غلظت 100 درصد، مهار بیشتری نسبت به عصاره خام انواع باکتری‌های بیماری‌زا، با تفاوت‌های آماری معنی‌دار نشان دادند (05/0P<).
نتیجه‌گیری: نانوذرات نقره تهیه‌شده از عصاره هگزانی بر روی جدایه‌های باکتری G-ve و G+Ve MDR (E. coli, P. aeruginosa, S. aureus, K. Pneumoniae, and E. faecalis) در غلظت‌های 100 میکروگرم بر میلی‌لیتر موثرتر از عصاره نانوذرات نقره Cladophora neglecta که بدون عصاره هگزان نانوذرات نقره تهیه شده بودند، مهار بسیار قابل توجهی را در همه گونه‌های باکتری نشان داد.

کلیدواژه‌ها


عنوان مقاله [English]

Antibacterial Activity of Silver Nanoparticles Synthesized by Hexane Extract of some freshwater algae Against multi-Drug Resistance Bacteria

نویسندگان [English]

  • Noor Khudair Saad
  • Ahmed Shaker Al Ashoor
Department of Biology, College of Science, University of Thi-Qar, Thi-Qar, 64001, Iraq
چکیده [English]

Objectives
Antibiotic-resistant bacteria have become a worldwide concern due to the unintentional use of antibiotics, which has resulted in bacterial strains resistant to many or all available antibiotics. The primary and secondary metabolites found in algae play a major role in the conversion of silver nitrate to silver nanoparticles (AgNPs).
 
Materials and methods
Hexane extract of some freshwater algae was used in the process of making these nanoparticles. The reaction solution's color changing from yellow to dark brown due to the surface plasmon resonance's excitation serves as evidence for this. AgNPs were identified using UV-Vis spectroscopy, proteins and phenols were found to play a significant role in the formation of AgNPs, according to research done using Fourier Transformation-infrared (FTIR) to identify the effective algae group that contributes to the formation of those nanoparticles. A scanning electron microscope (SEM) was used to characterize the shapes and sizes of the synthesized AgNPs, which included spherical, rod-like, and hexagonal structures. Vitek Compact 2 system-diagnosed Multi-Drug Resistant (MDR) bacteria were used to test AgNPs' antibacterial activity.
 
 
 
Results
A study was conducted on the antibacterial effectiveness of biosynthetic silver nanoparticles against selected isolates of MDR bacteria. The results showed that silver nanoparticles prepared from hexane extract of the isolated algae at a concentration of 100% showed greater inhibition than crude extract of all types of pathogenic bacteria, with statistically significant differences (P<0.05).
 
Conclusions
The silver nanoparticles prepared from hexane extract was more effective against G-ve and G+Ve MDR bacterial isolates (E. coli, P. aeruginosa, S. aureus, K. Pneumoniae, and E. faecalis) at concentrations 100 μg/mL than those prepared without silver nanoparticles hexane extract, the extract from Cladophora neglecta silver nanoparticles demonstrated highly significant inhibition in all species of bacteria.

کلیدواژه‌ها [English]

  • Antibacterial activity
  • Cladophora glomerata
  • freshwater algae
  • MDR
  • Silver nanoparticles
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