سنتز سبز نانوذرات فلزی اکسیدآهن و نقره از ریشه گیاه باریجه (Ferula Gummosa) و بررسی خصوصیات ضدمیکروبی آن‌ها

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه زیست شناسی، واحد اردبیل، دانشگاه آزاد اسلامی، اردبیل، ایران.

2 گروه شیمی، واحد اردبیل، دانشگاه آزاد اسلامی، اردبیل، ایران

3 گروه زیست شناسی ،واحد اردبیل، دانشگاه آزاد اسلامی، اردبیل، ایران

10.22103/jab.2025.24522.1636

چکیده

در این پژوهش ویژگی‌های نانوذرات فلزی هم‌چون اکسیدآهن و نقره سنتز سبز شده از عصاره آبی گیاه F. gummosa مورد مطالعه قرار گرفته و در ادامه به خواص ضدباکتریایی عصاره آبی حاصل از ریشه این گیاه و نانوذرات فلزی حاصل از آن پرداخته شد.
در این مطالعه سنتز سبز نانوذرات نقره و نانوذرات اکسید آهن با استفاده از عصاره گیاه باریجه صورت گرفت. هم‌چنین جهت بررسی مورفولوژی، اندازه و بار سطحی نانوذرات به ترتیب از میکروسکوپ الکترونی عبوری و دستگاه DLS استفاده شد. اثر ضدمیکروبی نانوذرات اکسیدآهن و نقره از دیگر بررسی‌های انجام شده در این تحقیق بود. بدین منظور ابتدا باکتر‌های Escherichia coli و Staphylococcus aureus بر روی محیط LB جامد کشت شدند. سپس غلظت‌های متفاوتی از نانوذرات (05/0، 1/0، 25/0، 5/0 و 1 میلی‌گرم) بر هر کدام از باکتری‌ها اثر داده شده و به مدت 24 ساعت در درجه حرارت ◦C 37 نگهداری شدند.
نتایج حاصل از طیف سنجی UV-Vis، FTIR و XRD نشان داد نانوذرات اکسید آهن و نقره با موفقیت از عصاره گیاه باریجه سنتز شده‌اند. تصاویر میکروسکوپ الکترونی عبوری از نانوذرات نقره سنتز شده از عصاره ریشه گیاه باریجه نشان داد که این نانوذرات دارای ساختار چند وجهی می‌باشند این درحالی بود که نانوذرات آهن از ساختار کروی برخوردار بودند. اندازه نانوذرات نقره و نانوذرات اکسید آهن به ترتیب برابر با 16 و 35 نانومتر بودند. میزان بار سطحی برای نانوذرات اکسید آهن برابر با mV 18- و برای نانوذرات نقره برابر با mV 23- بود. نتایج حاصل از بررسی خواص ضدمیکروبی ترکیبات نشان داد که نانوذرات نقره و عصاره گیاه باریجه از خواص ضدمیکروبی مطلوبی برخوردار بودند، با این حال اثر معنی‌داری برای خواص ضدمیکروبی نانوذرات اکسید آهن تا غلظت پایین تر از 1 میلی‌گرم مشاهده نشد. علاوه بر این نتایج حاصل از بررسی MIC و MBC از نانوذرات نقره و عصاره گیاه باریجه نشان داد. نانوذرات نقره به ترتیب در غلظت مورد استفاده 5/2 و 5 میلی گرم سبب مهار باکتر‌های E. coli و S. aureus می‌گردد. حداقل غلظت مورد نیاز جهت مهار باکتر‌های E. coli و S. aureus توسط عصاره گیاه باریجه 5 میلی‌گرم بر میلی‌لیتر بود.
طبق نتایج به دست آمده بین عصاره آبی حاصل از گیاه باریجه و نانوذرات نقره سنتز سبز شده از آن از نظر میزان فعالیت ضد-باکتریایی تفاوت معنی‌داری مشاهده شد. این در حالی است که خواص ضدباکتریایی عصاره آبی گیاه باریجه و نانوذرات نقره به طور معنی‌داری بیشتر از نانوذرات اکسیدآهن بود.

کلیدواژه‌ها

عنوان مقاله [English]

Green synthesis of iron oxide and silver metal nanoparticles from the roots of Ferula Gummosa and investigation of their antimicrobial properties

نویسندگان [English]

  • Hashem Yaghoubi 1
  • Somayyeh Rostamzadeh Mansour 2
  • Arman Ghorban Doost 3
1 Department of Biology, Ardabil Branch, Islamic Azad University, Ardabil, Iran.
2 Assistant Professor of Inorganic Chemistry, Islamic Azad University, Faculty of Chemistry, Ardabil, Iran
3 Department of Biology, Ardabil Branch, Islamic Azad University, Ardabil, Iran
چکیده [English]

Objective
Ferula gummosa is a stable, self-sufficient perennial herb of the Apiaceae family, known for its medicinal compounds such as flavonoids with antioxidant and anticancer properties. These compounds play a vital role in the green synthesis of metal nanoparticles due to their redox activity. This study focused on synthesizing iron oxide and silver nanoparticles using aqueous extract of F. gummosa and evaluating their antibacterial properties along with those of the plant extract.
Materials and methods
Green synthesis of silver and iron oxide nanoparticles was performed using F. gummosa extract. UV-Vis, FTIR, and XRD spectroscopy confirmed nanoparticle formation. Morphology, size, and surface charge were assessed using TEM and DLS. Antibacterial activity was tested against Escherichia coli and Staphylococcus aureus on solid LB medium using nanoparticle concentrations of 0.05, 0.1, 0.25, 0.5, and 1 mg, incubated at 37 °C for 24 hours.
Results
The results of UV-Vis, FTIR, and XRD spectroscopy showed that iron oxide and silver nanoparticles were successfully synthesized from the extract of the F. gummosa plant. TEM images showed silver nanoparticles had multifaceted structures while iron oxide nanoparticles were spherical. Their sizes were 16 nm and 35 nm, and surface charges were -23 mV and -18 mV, respectively. Antimicrobial tests revealed that both silver nanoparticles and the plant extract had effective antibacterial activity, whereas iron oxide nanoparticles showed negligible effects below 1 mg. MIC and MBC tests showed silver nanoparticles inhibited E. coli and S. aureus at 2.5 and 5 mg, respectively, while the plant extract required 5 mg/ml to inhibit both bacteria.
Conclusions
According to the results obtained, a significant difference was observed between the aqueous extract of the F. gummosa plant and the green synthetic silver nanoparticles prepared from it in terms of antibacterial activity. However, the antibacterial properties of the aqueous extract of the F. gummosa plant and silver nanoparticles were significantly higher than those of iron oxide nanoparticles.

کلیدواژه‌ها [English]

  • Ferula gammosa
  • iron oxide nanoparticles
  • Green synthesis
  • and silver nanoparticles

مراجع

Abedini, S., Sankian, M., Falak, R., Tehrani, M., Talebi, F., Shirazi, F. G., & Varasteh, A. R. (2011). An approach for detection and quantification of fruits' natural profilin: natural melon profilin as a model. Food and Agricultural Immunology, 22(1), 47-55. https://doi.org/10.1080/09540105.2010.524918
Ali, Z. A., Yahya, R., Sekaran, S. D., & Puteh, R. (2016). Green synthesis of silver nanoparticles using apple extract and its antibacterial properties. Advances in Materials Science and Engineering, 2016(1), 4102196.‏ https://doi.org/10.1155/2016/4102196
Azizi, S., Ahmad, M. B., Ibrahim, N. A., Hussein, M. Z., & Namvar, F. (2014). Cellulose nanocrystals/ZnO as a bifunctional reinforcing nanocomposite for poly (vinyl alcohol)/chitosan blend films: fabrication, characterization and properties. International Journal of Molecular Sciences, 15(6), 11040-11053.‏ https://doi.org/10.3390/ijms150611040
Babanejad, N., Farhadian, A., Omrani, I., & Nabid, M. R. (2017). Design, characterization and in vitro evaluation of novel amphiphilic block sunflower oil-based polyol nanocarrier as a potential delivery system: raloxifene-hydrochloride as a model. Materials Science and Engineering: C, 78, 59-68.‏ https://doi.org/10.1016/j.msec.2017.03.235
Bakkali, F., Averbeck, S., Averbeck, D., & Idaomar, M. (2008). Biological effects of essential oils–a review. Food and Chemical Toxicology, 46(2), 446-475.‏ https://doi.org/10.1016/j.fct.2007.09.106
Bourang, S., Noruzpour, M., Jahanbakhsh Godekahriz, S., Ebrahimi, H. A. C., Amani, A., Asghari Zakaria, R., & Yaghoubi, H. (2024b). Application of nanoparticles in breast cancer treatment: a systematic review. Naunyn-Schmiedeberg's Archives of Pharmacology, 397(9), 6459-6505.‏ https:// doi.org/ 10.1007/s00210-024-03082-y
Bourang, Sh., Jahanbakhsh-Godekahriz, S., Asghari-Zakaria, R., Parsa-Khankandi, H., & Noruzpour, M. (2024a). Green synthesis of iron oxide, copper, zinc oxide and silver nanoparticles from aqueous extract of F. vulgare and evaluation of their structural and antimicrobial properties. Agricultural Biotechnology Journal, 16(3), 61-88.‏ https://doi.org/10.22103/jab.2024.23102.1557
Carson, C. F., & Hammer, K. A. (2011). Chemistry and bioactivity of essential oils. Lipids and essential oils as antimicrobial agents, pp. 203-238.‏ https://doi.org/10.1002/9780470976623.ch9
Chokheli, V.A., P.A. Dmitriev, V.D. Rajput, S.D. Bakulin, A.S. Azarov, T.V. Varduni, V.V. Stepanenko, S. Tarigholizadeh, R.K. Singh & Verma, K.K. (2020). Recent development in micropropagation techniques for rare plant species. Plants, 9(12), 17-33. https://doi.org/10.3390/plants9121733
Cushnie, T. T., Cushnie, B., & Lamb, A. J. (2014). Alkaloids: An overview of their antibacterial, antibiotic-enhancing and antivirulence activities. International Journal of Antimicrobial Agents, 44(5), 377-386. https://doi.org/10.1016/j.ijantimicag.2014.06.001
Durán, N., Marcato, P. D., Alves, O. L., De Souza, G. I., & Esposito, E. (2005). Mechanistic aspects of biosynthesis of silver nanoparticles by several Fusarium oxysporum strains. Journal of Nanobiotechnology, 3, 1-7.‏ https://doi.org /10.1186/1477-3155-3-8
Edris, A. E. (2007). Pharmaceutical and therapeutic potentials of essential oils and their individual volatile constituents: a review. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives, 21(4), 308-323. https://doi.org/10.1002/ptr.2072
Ghorbani, A., Mohebbati, R., Jafarian, A. H., Vahedi, M. M., Hosseini, S. M., Soukhtanloo, M., & Sadeghnia, H. R. (2016). Toxicity evaluation of hydroalcoholic extract of Ferula gummosa root. Regulatory Toxicology and Pharmacology, 77, 35-41.‏ https://doi.org/10.1016/j.yrtph.2016.02.008
Gul, H. I., Ojanen, T., & Hänninen, O. (2002). Antifungal evaluation of bis Mannich bases derived from acetophenones and their corresponding piperidinols and stability studies. Biological and Pharmaceutical Bulletin, 25(10), 1307-1310. https://doi.org/10.1248/bpb.25.1307
He, Q., Liu, J., Sun, X., & Zhang, Z. R. (2004). Preparation and characteristics of DNA-nanoparticles targeting to hepatocarcinoma cells. World Journal of Gastroenterology, 10(5), 660. https://doi.org/10.3748/wjg.v10.i5.660
Heidarpour, A., Fourouzandeh-Shahraki, A. D., & Eghbalsaied, S. (2011). Effects of Spirulina platensis on performance, digestibility and serum biochemical parameters of Holstein calves. African Journal of Agricultural Research, 6(22), 5061-5065.‏ https://doi.org/10.5897/AJAR11.1076
Herlekar, M., Barve, S., & Kumar, R. (2014). Plant‐mediated green synthesis of iron nanoparticles. Journal of Nanoparticles, 2014(1), 140614.‏ https://doi.org/10.1155/2014/140614
Hussain, A. I., Anwar, F., Sherazi, S. T. H., & Przybylski, R. (2008). Chemical composition, antioxidant and antimicrobial activities of basil (Ocimum basilicum) essential oils depends on seasonal variations. Food Chemistry, 108(3), 986-995.‏ https://doi.org/10.1016/j.foodchem.2007.12.010
Jalali, H. T., Ebrahimian, Z. J., Evtuguin, D. V., & Neto, C. P. (2011). Chemical composition of oleo-gum-resin from Ferula gummosa. Industrial Crops and Products, 33(2), 549-553.‏ https://doi.org/10.1016/j.indcrop.2010.10.032
Mahboubi, M. (2016). Ferula gummosa, a traditional medicine with novel applications. Journal of Dietary Supplements, 13(6), 700-718. https://doi.org/10.3109/19390211.2016.1157715
Mahdavi, M., Namvar, F., Ahmad, M. B., & Mohamad, R. (2013). Green biosynthesis and characterization of magnetic iron oxide (Fe3O4) nanoparticles using seaweed (Sargassum muticum) aqueous extract. Molecules, 18(5), 5954-5964.‏ https://doi.org/10.3390/molecules18055954
Mehrsorosh, H., Gavanji, S., Larki, B., Mohammadi, M. D., Karbasiun, A., Bakhtari, A., & Mojiri, A. (2014). Essential oil composition and antimicrobial screening of some Iranian herbal plants on Pectobacterium carotovorum. Global Nest Journal, 16(2), 240-250.‏ https://www.researchgate.net/publication/263155216
Mohammadabadi, M. R., & Mozafari, M. R. (2018). Enhanced efficacy and bioavailability of thymoquinone using nanoliposomal dosage form. Journal of Drug Delivery Science and Technology, 47, 445-453. https://doi.org/10.1016/j.jddst.2018.08.019
Mohammadabadi, M. R., & Mozafari, M. R. (2019). Development of nanoliposome-encapsulated thymoquinone: Evaluation of loading efficiency and particle characterization. Journal of Biopharm, 11(4), 39-46. https://www.researchgate.net/publication/336881270
Mohammadabadi, M. R., El-Tamimy, M., Gianello, R., & Mozafari, M. R. (2009). Supramolecular assemblies of zwitterionic nanoliposome-polynucleotide complexes as gene transfer vectors: Nanolipoplex formulation and in vitro characterisation. Journal of Liposome Research, 19(2), 105-115.‏ https://doi.org/10.1080/08982100802547326
Mortazavi, F., Ericson, M., Story, D., Hulce, V. D., & Dunbar, G. L. (2005). Spatial learning deficits and emotional impairments in pentylenetetrazole-kindled rats. Epilepsy & Behavior, 7(4), 629-638. https://doi.org/10.1016/j.yebeh.2005.08.019
Murgueitio, E., Cumbal, L., Abril, M., Izquierdo, A., Debut, A., & Tinoco, O. (2018). Green synthesis of iron nanoparticles: Application on the removal of petroleum oil from contaminated water and soils. Journal of Nanotechnology, 2018(1), 4184769.‏ https://doi.org/10.1155/2018/4184769
Noruzpour, M., Asghari Zakaria, R., Zare, N., Ebrahimi, H. A., Parsa, H., & Bourang, S. (2024b). Investigating the Anticancer Properties of the Essential Oil and Aqueous Extract of Moringa oleifera and its Biosynthesized Metal Nanoparticles on MCF-7 and BT-549 Cell Lines. Iranian Journal of Breast Diseases, 17(1), 59-83.‏ http://ijbd.ir/article-1-1076-en.html
Noruzpuor, M., Asghari Zakaria, R., Zare, N., Ebrahimi, H. A., Parsa, H., & Bourang, S. (2024a). Green synthesis of metal nanoparticles using aqueous extract of Moringa oleifera L. and investigating their antioxidant and antibacterial properties. Applied Chemistry Today, 19(71), 283-302.‏ https://doi.org/10.22075/chem.2024.32548.2231
Owuama, C. I. (2017). Determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using a novel dilution tube method. African Journal of Microbiology Research, 11(23), 977-980.‏ https://www.researchgate.net/publication/317950371
Perumal Samy, R., & Gopalakrishnakone, P. (2010). Therapeutic potential of plants as anti‐microbials for drug discovery. Evidence‐Based Complementary and Alternative Medicine, 7(3), 283-294. https://doi.org/10.1093/ecam/nen036
Rahman, O., Mohapatra, S. C., & Ahmad, S. (2012). Fe3O4 inverse spinal super paramagnetic nanoparticles. Materials Chemistry and Physics, 132(1), 196-202. https://doi.org/10.1016/j.matchemphys.2011.11.032
Ramya, K., Mohan, R., Anupama, K. K., & Joseph, A. (2015). Electrochemical and theoretical studies on the synergistic interaction and corrosion inhibition of alkyl benzimidazoles and thiosemicarbazide pair on mild steel in hydrochloric acid. Materials Chemistry and Physics, 149, 632-647. https://doi.org/10.1016/j.matchemphys.2014.11.020
Routh, M. M., Raut, J. S., & Karuppayil, S. M. (2012). Dual properties of anticancer agents: an exploratory study on the in vitro anti-Candida properties of thirty drugs. Chemotherapy, 57(5), 372-380. https://doi.org/10.1159/000330454
Sadeghi, E., Almasi, A., Bashiri, M., & Mohammadi, M. (2016). Performance Evaluation of Alternative Disinfectants in Reducing Microbial Contamination of Vegetables Supplied from City of Kermanshah. Journal of Environmental Health Enginering, 3(2), 123-128.‏ http://jehe.abzums.ac.ir/article-1-225-en.html
Sohrabi-Gilani, N., Ghayournezhad, A., & Rostamzadeh Mansour, S. (2022). Determination of ultratrace levels of cobalt (II) and chromium (III) by magnetic dispersive solid-phase extraction (SPE) using urea-formaldehyde polymer/magnetite nanoparticles with flame atomic absorption spectrometry (FAAS). Analytical Letters, 55(16), 2650-2667.‏ https://doi.org/10.1080/00032719.2022.2067863
Villanueva-Ibáñez, M., Yañez-Cruz, M. G., Álvarez-García, R., Hernández-Pérez, M. A., & Flores-González, M. A. (2015). Aqueous corn husk extract–mediated green synthesis of AgCl and Ag nanoparticles. Materials Letters, 152, 166-169.‏ https://doi.org/10.1016/j.matlet.2015.03.097
Wiegand, I., Hilpert, K., & Hancock, R. E. (2008). Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nature Protocols, 3(2), 163-175. https://doi.org/10.1038/nprot.2007.521
Zarrabi, A., Alipoor Amro Abadi, M., Khorasani, S., Mohammadabadi, M. R., Jamshidi, A., Torkaman, S., ... & Rasti, B. (2020). Nanoliposomes and tocosomes as multifunctional nanocarriers for the encapsulation of nutraceutical and dietary molecules. Molecules, 25(3), 638.‏ https://doi.org/10.3390/molecules25030638
مجله بیوتکنولوژی کشاورزی
دوره 17، شماره 3
شهریور 1404
صفحه 1-26

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