تأثیر ترکیب کوئرستین-بربرین و عصاره میخک بر موش‌های آسمی القاشده با Ovalbumin

نوع مقاله : مقاله پژوهشی

نویسنده

بخش میکروبیولوژی، دانشکده دامپزشکی، دانشگاه بغداد، استان بغداد، عراق.

10.22103/jab.2025.25794.1755

چکیده

هدف: آسم یک اختلال التهابی مزمن مجاری هوایی است که با بیش‌واکنشی و تغییرات ساختاری شناخته می‌شود و عمدتاً ناشی از بی‌نظمی ایمنی است. ترکیبات طبیعی مانند بربرین، کوئرستین و عصاره میخک دارای ویژگی‌های ضدالتهابی و آنتی‌اکسیدانی مستند هستند. این پژوهش با هدف بررسی واکنش‌های ایمنی‌تعدیل‌گر و حفاظتی ترکیب کوئرستین–بربرین و عصاره میخک در الگوی آسم القاشده با اووالبومین (OVA) در موش‌ها انجام شد.
مواد و روش‌ها: سی موش سوییس از هر دو جنس به‌صورت تصادفی به سه گروه (۱۰ موش در هر گروه) تقسیم شدند. گروه اول دوز خوراکی ترکیب کوئرستین–بربرین (۱۰۰ میلی‌گرم بر میلی‌لیتر، 1/0 میلی‌لیتر) را یک‌بار در هفته به‌مدت ۸ هفته دریافت کرد. گروه دوم با همان رژیم درمانی عصاره میخک دریافت نمود. گروه سوم به‌عنوان کنترل مثبت در روز اول و مجدداً پس از ۸ هفته 10 درصد OVA (3/0 میلی‌لیتر، به‌صورت داخل صفاقی) برای القای آسم دریافت کرد. در پایان دوره درمان، نمونه‌های خون و سرم جهت سنجش شمارش گلبول‌های سفید (WBC)، ایمونوگلوبولین E (IgE) و اینترلوکین-۴ (IL-4) به روش ELISA جمع‌آوری شد. سپس تمام گروه‌های آزمایشی پیش از euthanasia در هفته نهم مجدداً با OVA (3/0 میلی‌لیتر، داخل صفاقی) تیمار شدند. در ادامه بررسی آسیب‌شناسی بافتی ریه و نای انجام گرفت.
نتایج: درمان با ترکیب کوئرستین-بربرین یا عصاره میخک موجب کاهش معنادار شمارش WBC، سطح IL-4 و IgE در مقایسه با گروه کنترل مثبت گردید (p ≤ 0.05). تحلیل آسیب‌شناسی بافتی کاهش چشمگیری در التهاب راه‌های هوایی، نفوذ سلولی و تخریب بافتی در گروه‌های درمانی نشان داد که بیانگر کاهش پاتولوژی ریوی ناشی از OVA بود. هر دو درمان واکنش‌های حفاظتی مشابهی بروز دادند و شواهدی از بازسازی یکپارچگی بافتی در مقایسه با گروه‌های درمان‌نشده مشاهده شد.
نتیجه‌گیری: نتایج نشان می‌دهد که ترکیب کوئرستین-بربرین و عصاره میخک فعالیت ایمنی‌تعدیل‌گر قوی داشته و پاسخ‌های التهابی و تخریب بافتی در آسم القاشده با OVA را کاهش می‌دهند. این یافته‌ها پتانسیل درمانی ترکیبات گیاهی را به‌عنوان راهکارهای کمکی یا جایگزین در مدیریت التهاب مجاری هوایی و آسم برجسته می‌سازند. برای روشن شدن سازوکارهای مولکولی زمینه‌ای و بررسی اهمیت بالینی آن‌ها تحقیقات بیشتری لازم است.

کلیدواژه‌ها

عنوان مقاله [English]

The impact of Quercetin-Berberine compound and Clove extract on Ovalbumin-induced asthmatic mice

نویسنده [English]

  • Roua Jassim
Department. Of Microbiology, College of Veterinary Medicine, University of Baghdad, Baghdad province, Iraq.
چکیده [English]

Objective
Asthma is a chronic inflammatory disorder of the airways described by hyperresponsiveness and constructional remodeling, carried mostly by immune dysregulation. Indigenous combinations as berberine, quercetin, and clove extractive contain well-documented anti-inflammatory and antioxidant attributes. This investigation targeted to survey the immunomodulatory and protective reactions of a quercetin–berberine compound and clove extractive in an ovalbumin (OVA)-induced murine pattern of asthma.
Materials and Methods
Thirty Swiss mice of both sexes were randomly assigned to three groups (n = 10 per group). The first group obtained an oral dose of quercetin–berberine compound (100 mg/mL, 0.1 mL) once a week for 8 weeks. The second group was given treatment with clove extractive applying the same regimen. The third group acted as a positive control and was managed 10% OVA (0.3 mL, intraperitoneally) on day one and anew after 8 weeks to incite asthma. At the termination of the treatment phase, blood and serum samples were gathered for quantification of white blood cells (WBCs), immunoglobulin E (IgE), and interleukin-4 (IL-4), applying ELISA. All experimental groups were thereafter treated with OVA (0.3 mL, intraperitoneally) before euthanasia at week nine. Histopathological examination of the lungs and trachea was then carried out.
Results
Treatment with either quercetin–berberine compound or clove extractive meaningfully decreased WBC counts, IL-4, and IgE levels in comparison to the positive control group (p ≤ 0.05). Histopathological analysis disclosed a marked depletion in airway inflammation, cellular infiltration, and tissue destruction in the treatment groups, denoting an attenuation of OVA-incited pulmonary pathology. Both treatments revealed equivalent protective reactions, with evidence of restored tissue integrity in comparison to untreated controls.
Conclusions
The results offer that the quercetin–berberine compound and clove extractive exert potent immunomodulatory activity, alleviating inflammatory responses and tissue destruction in OVA-incited asthma. These outcomes emboss the therapeutic potential of plant-derived combinations as adjunctive or alternative plans in the administration of airway inflammation and asthma. Further investigations are warranted to clarify underlying molecular mechanisms and assess their clinical relevance.

کلیدواژه‌ها [English]

  • Asthma
  • Clove extractive
  • ELISA
  • Immune modulation
  • Ovalbumin

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مجله بیوتکنولوژی کشاورزی
دوره 17، شماره 3
شهریور 1404
صفحه 385-406

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