همبستگی پلی‌مورفیسم ژن ACE (I/D) با دیابت نوع ۲ و دوره پس از بهبودی از کووید-۱۹ در بیماران عراقی: یک مطالعه موردی-شاهدی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه شیمی و بیوشیمی، دانشکده پزشکی، دانشگاه بابل، عراق

2 دانشکده علوم، دانشگاه القاسم سبز، بابل، عراق.

3 فناوری بیهوشی، دانشکده فناوری‌های پزشکی سلامت، دانشگاه اوروک، عراق.

4 دانشکده دامپزشکی، دانشگاه القاسم سبز، بابل، عراق.

چکیده

هدف: دیابت نوع ۲ (T2DM) یک اختلال متابولیک مزمن است که با افزایش سطح قند خون به‌دلیل مقاومت به انسولین، کاهش عملکرد سلول‌های بتای پانکراس یا هر دو مشخص می‌شود. ویروس SARS-CoV-2 نیز یک عامل بیماری‌زای تنفسی است که موجب بیماری کووید-۱۹ می‌گردد. هدف از این مطالعه، بررسی ارتباط بین سطوح پارامترهای FBS، HbA1c، WBC، درصد لنفوسیت‌ها، T3، T4، TSH، CRP، D-dimer، LDH و فریتین با پلی‌مورفیسم ژن ACE (I/D) در بیماران مبتلا به دیابت نوع ۲ (G2) و افراد بهبود‌یافته از کووید-۱۹ (G3) در مقایسه با گروه کنترل (G1) بود.
مواد و روش‌ها: در این مطالعه، ۶۰ بیمار مبتلا به دیابت نوع ۲ (G2)، ۶۰ بیمار بهبود‌یافته از کووید-۱۹ (G3) و ۶۰ فرد سالم هم‌نژاد به‌عنوان گروه کنترل (G1) شرکت داشتند. سن تمامی شرکت‌کنندگان بین ۳۰ تا ۴۵ سال بود و همگی به بیمارستان آموزشی دیوانیه در استان دیوانیه عراق مراجعه کرده بودند. پارامترهای بیوشیمیایی شامل FBS، HbA1c، WBC، درصد لنفوسیت‌ها، T3، T4، TSH، CRP، D-dimer، LDH و فریتین اندازه‌گیری شد. همچنین، پلی‌مورفیسم ژن ACE (I/D) با استفاده از روش PCR تکثیر و بررسی گردید.
نتایج: نتایج مطالعه نشان داد که بین پارامترهای بیوشیمیایی مورد بررسی و پلی‌مورفیسم ژن ACE ارتباط معناداری وجود دارد. اختلاف آماری معنی‌داری (P < 0.05) بین ژنوتیپ‌های II و ID در مقایسه با ژنوتیپ DD در بیمارانی که در معرض خطر بالای ابتلا به دیابت نوع ۲ پس از بهبودی از کووید-۱۹ قرار داشتند، مشاهده شد. ژنوتیپ‌های II و ID با بروز بالاتر دیابت نوع ۲ در افراد عراقی بهبود‌یافته از کووید-۱۹ همراه بودند.
نتیجه‌گیری: نتایج این مطالعه نشان می‌دهد که پلی‌مورفیسم ژن ACE (I/D) به‌طور معناداری با تغییرات پارامترهای بیوشیمیایی در بیماران مبتلا به دیابت نوع ۲ و افراد بهبود‌یافته از کووید-۱۹ مرتبط است. خطر بروز یا تشدید دیابت نوع ۲، به‌ویژه در بازماندگان کووید-۱۹، در حاملان ژنوتیپ‌های II و ID بیشتر از ژنوتیپ DD بود. این نتایج بیانگر آن است که ترکیب ژنتیکی سیستم رنین-آنژیوتانسین می‌تواند در حساسیت متابولیک پس از عفونت SARS-CoV-2 نقش داشته باشد و به‌عنوان یک شاخص بالقوه در غربالگری، پیشگیری و مدیریت بهتر بیماران پرخطر مورد استفاده قرار گیرد. با این حال، انجام مطالعات گسترده‌تر در جمعیت‌های بزرگ‌تر برای تأیید این یافته‌ها ضروری است.

کلیدواژه‌ها

عنوان مقاله [English]

Correlation of ACE (I/D) gene polymorphisms with type 2 diabetes mellitus and post-recovery from Covid-19 in Iraqi patients: A case-control investigation

نویسندگان [English]

  • Tariq Hussien Mgheer 1
  • Rawaa S. A. Al-Azawi 2
  • Wafaa Zekyer Mohi 3
  • Hamzah H. K. Al-Shukri 4
1 Department of Chemistry and Biochemistry, College of Medicine, University of Babylon, Iraq
2 College of Science, Al-Qasim Green University, 51013, Babylon, Iraq.
3 Anesthetic Technology, College of health medical technology, Uruk university, Iraq.
4 College of Veterinary Medicine, Al-Qasim Green University,51013, Babylon, Iraq.
چکیده [English]

Objective
Type 2 diabetes mellitus (T2DM) is a chronic metabolic disorder. This disorder causes elevated blood sugar levels due to either insulin resistance, decreased pancreatic beta cell function, or both. SARS-CoV-2 is also a pathogenic respiratory virus that causes COVID-19. The aim of this study was to study the correlation between the levels of FBS, HbA1c, WBC, lymphocyte percentage, T3, T4, TSH, CRP, D-dimer, LDH, and ferritin parameters and ACE gene polymorphism (I/D) in patients with type 2 diabetes (G2) and recovered COVID-19 (G3) compared to the control group (G1).
Materials and methods
This study included 60 patients with type 2 diabetes (G2), 60 recovered patients from COVID-19 (G3), and 60 ethnically matched controls (G1). All subjects were between 30 and 45 years of age. All subjects were referred to Diwaniyah Teaching Hospital in Diwaniyah Province, Iraq. Biochemical parameters FBS, HbA1c, WBC, lymphocyte percentage, T3, T4, TSH, CRP, D-dimer, LDH, and ferritin were calculated. PCR was also used to amplify and examine the ACE(I/D) gene polymorphism.
Results
The study found a significant relation among the parameters (FBS, HbA1c, WBC, Lymphocyte%, T3, T4, TSH, CRP, D-dimer, LDH, and ferritin) and ACE gene polymorphisms. The results showed that there was a significant difference (P < 0.05) between genotypes II and ID compared to genotype DD in patients at high risk of developing type 2 diabetes in COVID-19 recoveries. Genotypes II and ID had a higher incidence of type 2 diabetes in Iraqi individuals who recovered from COVID-19.
Conclusion
The findings of this study showed that the ACE gene polymorphism (I/D) was significantly associated with changes in biochemical parameters in patients with type 2 diabetes and recovered patients from COVID-19. The risk of developing or worsening type 2 diabetes, especially in recovered patients from COVID-19, was higher for genotypes II and ID than for genotype DD. These results indicate that the genetic composition of the renin-angiotensin system may play a role in metabolic susceptibility after SARS-CoV-2 infection. Therefore, it may be used as a potential indicator in screening, prevention, and better management of patients at risk. However, larger studies in larger populations are needed to confirm these findings.

کلیدواژه‌ها [English]

  • ACE
  • Covid-19
  • type 2 diabetes mellitus disease
  • T2DM

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مجله بیوتکنولوژی کشاورزی
دوره 18، شماره 1
فروردین 1405
صفحه 281-300

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