مجله بیوتکنولوژی کشاورزی

مجله بیوتکنولوژی کشاورزی

فعالیت ضدباکتریایی نانوکامپوزیت ZnO-chitosan سنتزشده به روش لیزر علیه Pseudomonas aeruginosa مقاوم به چند دارو جداشده از دهان و اثر لاروکشی آن بر Culex pipiens

نوع مقاله : مقاله پژوهشی

نویسندگان
1 گروه زیست‌شناسی، دانشکده علوم برای بانوان، دانشگاه بابل، عراق
2 گروه زیست‌شناسی، دانشکده علوم برای بانوان، دانشگاه بابل، عراق.
3 گروه لیزر، دانشکده علوم برای بانوان، دانشگاه بابل، عراق.
4 گروه زیست‌فناوری پزشکی، دانشکده علوم، دانشگاه المستقبل، بابل 51001، عراق.
10.22103/jab.2026.27253.1917
چکیده
هدف: بیماری‌های منتقل‌شونده توسط پشه‌ها و همچنین باکتری‌های مقاوم به چند دارو، از مهم‌ترین تهدیدهای سلامت عمومی به شمار می‌روند. این مطالعه با هدف ارزیابی فعالیت ضدباکتریایی نانوکامپوزیت ZnO-chitosan سنتزشده به روش لیزری علیه جدایه‌های دهانی Pseudomonas aeruginosa مقاوم به چند دارو و نیز بررسی فعالیت لاروکشی آن علیه پشه Culex pipiens انجام شد.
مواد و روش‌ها: تعداد 30 جدایه سودوموناس از حفره دهانی بیماران مراجعه‌کننده به بیمارستان حله جمع‌آوری شد. برای شناسایی و تفکیک باکتری‌ها از سیستم VITEK استفاده گردید و آزمون انتشار دیسک برای تعیین حساسیت آنتی‌بیوتیکی انجام شد. همچنین مقادیر حداقل غلظت مهارکنندگی (MIC) با استفاده از روش رقت‌سازی میکروبی در محیط مایع (Broth Microdilution) مطابق دستورالعمل‌های مؤسسه استانداردهای بالینی و آزمایشگاهی (CLSI) تعیین گردید.
نتایج: از مجموع 30 جدایه بررسی‌شده، 20 جدایه به عنوان Pseudomonas aeruginosa شناسایی شدند. میزان مقاومت آنتی‌بیوتیکی در این جدایه‌ها برای تیکارسیلین-کلاوولانیک اسید (TCC) برابر 50 درصد، برای مروپنم (MRP) برابر 85 درصد و برای سفتازیدیم (CAZ) برابر 75 درصد بود. تمامی 20 جدایه دارای ژن GyrA1 بودند. نانوکامپوزیت ZnO-chitosan دارای قطر هاله عدم رشد 20 میلی‌متر و حداقل غلظت مهارکنندگی (MIC) برابر با 5/12 میکروگرم بر میلی‌لیتر بود. اثر حشره‌کشی نانوکامپوزیت ZnO-Cs بر میزان مرگ‌ومیر تخم‌ها در دو زمان مواجهه 24 و 48 ساعت مورد ارزیابی قرار گرفت. تحلیل آماری نشان داد که مدت زمان مواجهه تأثیر معنی‌داری بر میزان مرگ‌ومیر تخم‌ها دارد. همچنین میزان مرگ‌ومیر لاروها با افزایش زمان مواجهه افزایش یافت؛ به‌طوری که پس از 48 ساعت تیمار، میزان مرگ‌ومیر در مراحل اول تا چهارم لاروی به ترتیب 90%، 5/80%، 4/78% و 34/20% بود، در حالی که پس از 24 ساعت مواجهه این مقادیر به ترتیب 4/80%، 09/61%، 04/52% و 10% گزارش شد.
نتیجه‌گیری: نانوکامپوزیت ZnO-chitosan فعالیت ضدباکتریایی قابل توجهی علیه Pseudomonas aeruginosa مقاوم به چند دارو و همچنین اثرات لاروکشی قوی علیه Culex pipiens از خود نشان داد. این یافته‌ها بیانگر پتانسیل این نانوکامپوزیت به عنوان یک عامل ضدمیکروبی سازگار با محیط زیست و نیز یک راهکار مؤثر برای کنترل ناقلان بیماری است.
کلیدواژه‌ها

عنوان مقاله English

Antibacterial activity of laser-ablated ZnO-chitosan nanocomposite against multidrug-resistant oral Pseudomonas aeruginosa and its larvicidal effect on culex pipiens

نویسندگان English

Tsahel H. Al-Dulaimi 1
Nisreen Kaddim Radi 2
Nagham M. Obaid 3
Hasanain Khaleel Shareef 4
Israa Adnan Ibraheam 2
Nebras Mohammed Sahi 2
Oras Abdulsayed Mahdi 1
Zeana Shaker AL-Hindi 1
Raad A Kadhim 1
1 Department of Biology, College of Science for Women, University of Babylon, Iraq.
2 Department of Biology, College of Science for Women, University of Babylon, Iraq.
3 Department of Laser, College of Science for Women, University of Babylon, Iraq.
4 Department of Medical Biotechnology, College of Science, Al-Mustaqbal University, 51001, Babylon, Iraq
چکیده English

Abstract
Objective
Diseases transmitted by mosquitoes and bacteria that are resistant to multiple drugs are serious health threats. This study aimed to evaluate the antibacterial activity of a laser-synthesized ZnO-chitosan nanocomposite against oral isolates of multidrug-resistant Pseudomonas aeruginosa and its larvicidal activity against Culex pipiens.
Materials and methods
Thirty Pseudomonas isolates were obtained from the oral cavities of patients at Hilla Hospital. We utilized the VITEK system to separate the bacteria and disc diffusion tests to find out how well they respond to antibiotics. The MIC values were determined using the broth microdilution method according to CLSI guidelines.
Results
Pseudomonas was positive for 20 of the 30 isolates. Antibiotic resistance was 50% for Ticarcillin-Clavulanic Acid (TCC), 85% for Meropenem (MRP), and 75% for Ceftazidime (CAZ). All of the 20 isolates showed positive for the GyrA1 gene; the ZnO-chitosan nanocomposite had an inhibition zone diameter of 20 mm with a 12.5 μg/mL minimum inhibitory concentration (MIC). The insecticidal effect of ZnO-Cs nanocomposite on egg mortality rates at two different exposure times (24 and 48 hours). Statistical analysis demonstrated a significant effect of exposure time on egg mortality rates. While larval mortality rates increased with increasing exposure time, reaching 90%, 80.5%, 78.4%, and 20.34% after 48 hours of treatment from the first to the fourth larval stage, respectively, compared to 80.4%, 61.09%, 52.04%, and 10% after only 24 hours from the first to the fourth larval stage.
Conclusion
The ZnO-chitosan nanocomposite exhibited significant antibacterial activity against multidrug-resistant P. aeruginosa and strong larvicidal effects against C. pipiens, suggesting its potential as an eco-friendly antimicrobial and vector-control agent.
The ZnO-chitosan nanocomposite exhibited significant antibacterial activity against multidrug-resistant P. aeruginosa and strong larvicidal effects against C. pipiens, suggesting its potential as an eco-friendly antimicrobial and vector-control agent.
Keywords: P. aeruginosa, ZnO-Cs nanocomposite, laser ablation, AST test, Dental plaque

کلیدواژه‌ها English

P. aeruginosa
ZnO-Cs nanocomposite
laser ablation
AST test
Dental plaque
Abbott, W. S. (1925). A method of computing the effectiveness of an insecticide. Journal of Economic Entomology, 18(2), 265-267. https://doi.org/10.1093/jee/18.2.265a
Acharya, S., & Barik, T. K. (2025). Larvicidal and pupicidal activity of neonicotinoid insecticide imidacloprid against southern house mosquito, Culex quinquefasciatus (Say) (Diptera: Culicidae). Acta Parasitologica, 70(4), Article 171. https://doi.org/10.1007/s11686-025-01112-0
Agarwal, H., Kumar, S. V., & Rajeshkumar, S. (2017). A review on green synthesis of zinc oxide nanoparticles - An eco-friendly approach. Resource-Efficient Technologies, 3(4), 406-413. https://doi.org/10.1016/j.reffit.2017.03.002
Ali, H., Ismail, A. M., & Menazea, A. A. (2022). Multifunctional Ag/ZnO/chitosan ternary bio-nanocomposites synthesized via laser ablation with enhanced optical, antibacterial, and catalytic characteristics. Journal of Water Process Engineering, 49, Article 102940. https://doi.org/10.1016/j.jwpe.2022.102940
Aouadi, A., Hamada Saud, D., Rebiai, A., Achouri, A., Benabdesselam, S., Mohamed Abd El-Mordy, F., Pohl, P., Ahmad, S. F., Attia, S. M., Abulkhair, H. S., Ararem, A., & Messaoudi, M. (2024). Introducing the antibacterial and photocatalytic degradation potentials of biosynthesized chitosan, chitosan-ZnO, and chitosan-ZnO/PVP nanoparticles. Scientific Reports, 14(1), Article 14753. https://doi.org/10.1038/s41598-024-65579-z
Buzea, C., Pacheco, I. I., & Robbie, K. (2007). Nanomaterials and nanoparticles: Sources and toxicity. Biointerphases, 2(4), MR17-MR71. https://doi.org/10.1116/1.2815690
Chinnathambi, A., Ali Alharbi, S., Lavarti, R., Jhanani, G. K., On-Uma, R., Jutamas, K., & Anupong, W. (2023). Larvicidal and pupicidal activity of phyto-synthesized zinc oxide nanoparticles against dengue vector Aedes aegypti. Environmental Research, 216(Pt 2), Article 114574. https://doi.org/10.1016/j.envres.2022.114574
Czyżowska, A., & Barbasz, A. (2022). A review: Zinc oxide nanoparticles - friends or enemies? International Journal of Environmental Health Research, 32(4), 885-901. https://doi.org/10.1080/09603123.2020.1805415
Divya Bharathi, J., & Suseem, S. R. (2024). Larvicidal activity of CuO and ZnO nanoparticles against Aedes aegypti and Anopheles stephensi mosquito vectors—A greener approach by Phaseolus vulgaris L. aqueous extract as bio-reductant. Results in Chemistry, 7, Article 101408. https://doi.org/10.1016/j.rechem.2024.101408
Elemike, E. E., Onwudiwe, D. C., & Mbonu, J. I. (2021). Green synthesis, structural characterization and photocatalytic activities of chitosan-ZnO nano‐composite. Journal of Inorganic and Organometallic Polymers and Materials, 31, 3356-3367. https://doi.org/10.1007/s10904-021-01988-1
El-Telbany, M., & El-Sharaki, A. (2022). Antibacterial and anti-biofilm activity of silver nanoparticles on multi-drug resistance Pseudomonas aeruginosa isolated from dental-implant. Journal of Oral Biology and Craniofacial Research, 12(1), 199-203. https://doi.org/10.1016/j.jobcr.2021.12.002
Gunathilaka, U. M. T. M., de Silva, W. A. P. P., Dunuweera, S. P., & Rajapakse, R. M. G. (2021). Effect of morphology on larvicidal activity of chemically synthesized zinc oxide nanoparticles against mosquito vectors. RSC Advances, 11(15), 8857-8866. https://doi.org/10.1039/D1RA00014D
Heidarpour, F., Mohammadabadi, M. R., Zaidul, I. S. M., Maherani, B., Saari, N., Hamid, A. A., Abas, F., Manap, M. Y. A., & Mozafari, M. R. (2011). Use of prebiotics in oral delivery of bioactive compounds: A nanotechnology perspective. Pharmazie, 66(5), 319-324. https://doi.org/10.1691/ph.2011.0279
Hemingway, J., & Ranson, H. (2000). Insecticide resistance in insect vectors of human disease. Annual Review of Entomology, 45, 371-391. https://doi.org/10.1146/annurev.ento.45.1.371
Hussein, A. A., Kaddim Radi, N., & Mohammed Sahi, N. (2025). Integrative computational and experimental study of propolis, polyvinyl alcohol, and Alhagi maurorum complex as anticancer and antibacterial agents. Journal of Biomaterials Science, Polymer Edition, 36(12), 1718-1748. https://doi.org/10.1080/09205063.2025.2464448
Ibrahim, A. M. A., Thabet, M. A., & Ali, A. M. (2023). Physiological and developmental dysfunctions in the dengue vector Culex pipiens (Diptera: Culicidae) immature stages following treatment with zinc oxide nanoparticles. Pesticide Biochemistry and Physiology, 192, Article 105395. https://doi.org/10.1016/j.pestbp.2023.105395
Jiang, J., Pi, J., & Cai, J. (2018). The advancing of zinc oxide nanoparticles for biomedical applications. Bioinorganic Chemistry and Applications, 2018, Article 1062562. https://doi.org/10.1155/2018/1062562
Konkuri, M., Kharrazi, S., Erfani, Y., Haghighat, S. (2024). Antibacterial and antibiofilm effect of zinc oxide nanoparticles on P. aeruginosa variants isolated from young patients with cystic fibrosis. Microbial Pathogenesis, 195, Article 106854. https://doi.org/10.1016/j.micpath.2024.106854
Lasserre, J. F., Brecx, M. C., & Toma, S. (2018). Oral microbes, biofilms and their role in periodontal and peri-implant diseases. Materials, 11(10), Article 1802. https://doi.org/10.3390/ma11101802
Mandal, A. K., Katuwal, S., Tettey, F., Gupta, A., Bhattarai, S., Jaisi, S., Bhandari, D. P., Shah, A. K., Bhattarai, N., & Parajuli, N. (2022). Current research on zinc oxide nanoparticles: Synthesis, characterization, and biomedical applications. Nanomaterials, 12(17), Article 3066. https://doi.org/10.3390/nano12173066
Mekkawy, I. A., Mahmoud, U. M., Hana, M. N., & Sayed, A. E. H. (2019). Cytotoxic and hemotoxic effects of silver nanoparticles on the African catfish, Clarias gariepinus (Burchell, 1822). Ecotoxicology and Environmental Safety, 171, 638-646. https://doi.org/10.1016/j.ecoenv.2019.01.011
Mohammadabadi, M. R., & Mozafari, M. R. (2018). Enhanced efficacy and bioavailability of thymoquinone using nanoliposomal dosage form. Journal of Drug Delivery Science and Technology, 47, 445-453. https://doi.org/10.1016/j.jddst.2018.08.019
Mohammed, Y. H., Holmes, A., Haridass, I. N., Sanchez, W. Y., Studier, H., Grice, J. E., Benson, H. A. E., & Roberts, M. S. (2019). Support for the safe use of zinc oxide nanoparticle sunscreens: Lack of skin penetration or cellular toxicity after repeated application in volunteers. Journal of Investigative Dermatology, 139(2), 308-315. https://doi.org/10.1016/j.jid.2018.08.024
Mostafa, W. A., Abdel-Raoof, A. M., Attala, K., & Elgazzar, E. (2021). Enhancement the larvicidal activity of nanostructure copper oxide against Culex pipiens mosquito by yttrium replacement based on crystallite size reduction and topographic surface nature. Materials Research Express, 8(11), Article 115006. https://doi.org/10.1088/2053-1591/ac343d
Nguyen, K. V., Nguyen, T. V., Nguyen, H. T. T., & Le, D. V. (2018). Mutations in the gyrA, parC, and mexR genes provide functional insights into the fluoroquinolone-resistant Pseudomonas aeruginosa isolated in Vietnam. Infection and Drug Resistance, 11, 275-282. https://doi.org/10.2147/IDR.S147581
Nouri, R., Ahangarzadeh Rezaee, M., Hasani, A., Aghazadeh, M., & Asgharzadeh, M. (2016). The role of gyrA and parC mutations in fluoroquinolones-resistant Pseudomonas aeruginosa isolates from Iran. Brazilian Journal of Microbiology, 47(4), 925-930. https://doi.org/10.1016/j.bjm.2016.07.016
Prokhorov, E., Luna-Bárcenas, G., Yáñez Limón, J. M., Gómez Sánchez, A., & Kovalenko, Y. (2020). Chitosan-ZnO nanocomposites assessed by dielectric, mechanical, and piezoelectric properties. Polymers, 12(9), Article 1991. https://doi.org/10.3390/polym12091991
Radi, N. K., & Al-Marzoqi, A. H. (2023). The distribution of antimicrobial resistance and the presence of virulence genes in Escherichia coli isolated from frozen chicken meat in Iraq. AIP Conference Proceedings, 2776, Article 020003. https://doi.org/10.1063/5.0136728
Radi, N. K., Al-Daher, R., & Kadhim, H. (2025a). A novel antibacterial and anticancer property of Iraqi honey bee venom. International Research Journal of Multidisciplinary Scope, 6(4), 1378-1392. https://doi.org/10.47857/irjms.2025.v06i04.07982
Radi, N. K., Mohammed, H. A., Alwarid, R. J., Mohammed, N. A., & Ewadh, R. M. J. (2025b). Polyvinylpyrrolidone-loaded Zn and ZnO nanoparticles influence Escherichia coli isolated from urinary tract infection patients. Pharmakeftiki, 37(2S).
Sada, M., Kimura, H., Nagasawa, N., Akagawa, M., Okayama, K., Shirai, T., Sunagawa, S., Kimura, R., Saraya, T., Ishii, H., Kurai, D., Tsugawa, T., Nishina, A., Tomita, H., Okodo, M., Hirai, S., Ryo, A., Ishioka, T., & Murakami, K. (2022). Molecular evolution of the Pseudomonas aeruginosa DNA gyrase gyrA gene. Microorganisms, 10(8), Article 1660. https://doi.org/10.3390/microorganisms10081660
Sedghi, L., DiMassa, V., Harrington, A., Lynch, S. V., & Kapila, Y. L. (2021). The oral microbiome: Role of key organisms and complex networks in oral health and disease. Periodontology 2000, 87(1), 107-131. https://doi.org/10.1111/prd.12393
Sharma, S., Mohler, J., Mahajan, S. D., Schwartz, S. A., Bruggemann, L., & Aalinkeel, R. (2023). Microbial biofilm: A review on formation, infection, antibiotic resistance, control measures, and innovative treatment. Microorganisms, 11(6), Article 1614. https://doi.org/10.3390/microorganisms11061614
Siddiqi, K. S., ur Rahman, A., Tajuddin, & Husen, A. (2018). Properties of zinc oxide nanoparticles and their activity against microbes. Nanoscale Research Letters, 13, Article 141. https://doi.org/10.1186/s11671-018-2532-3
Souza, R., Haberbeck, L., Riella, H., Ribeiro, D., & Carciofi, B. (2019). Antibacterial activity of zinc oxide nanoparticles synthesized by solochemical process. Brazilian Journal of Chemical Engineering, 36, 885-893. https://doi.org/10.1590/0104-6632.20190362s20180027
Thanh, V. M., Huong, N. T., Nam, D. T., Dung, N. D. T., Le Van, T., & Nguyen-Le, M.-T. (2020). Synthesis of ternary Fe₃O₄/ZnO/chitosan magnetic nanoparticles via an ultrasound-assisted coprecipitation process for antibacterial applications. Journal of Nanomaterials, 2020, Article 8875471. https://doi.org/10.1155/2020/8875471
Yonezawa, M., Takahata, M., Matsubara, N., Watanabe, Y., & Narita, H. (1995). DNA gyrase gyrA mutations in quinolone-resistant clinical isolates of Pseudomonas aeruginosa. Antimicrobial Agents and Chemotherapy, 39(9), 1970-1972. https://doi.org/10.1128/AAC.39.9.1970
Zhao, C.-X., He, L., Qiao, S. Z., & Middelberg, A. P. J. (2011). Nanoparticle synthesis in microreactors. Chemical Engineering Science, 66(7), 1463-1479. https://doi.org/10.1016/j.ces.2010.08.039

مقالات آماده انتشار، پذیرفته شده
انتشار آنلاین از 31 مرداد 1405