شناسایی ژنتیکی و طبقه بندی آرتمیای ایران با استفاده از نشانگرهای ریزماهواره

نوع مقاله : مقاله پژوهشی

نویسندگان

چکیده

در این پژوهش، تنوع ژنتیکی جمعیت آرتمیا اورمیانا و آرتمیا فرانسیسکانا­ی موجود در ایران با استفاده از 5 جفت آغازگر ریزماهواره­ای (Af-B105TAIL، Af-A136، Apdq03TAIL، Apdq04TAIL و Apdq05TAIL) ویژه­ی آرتمیا فرانسیسکانا و آرتمیا پارتنوژنتیکا بررسی شد. DNA ژنومی 50 سیست آرتیمیا از هر جمعیت به صورت انفرادی و با روش گلوله­ی داغ استخراج شد. محصولات PCR با استفاده از 5 جفت آغازگر با موفقیت تکثیر و فرآورده­ها­ی حاصل بر روی ژل پلی اکریل آمید واسرشته­ساز 6% الکتروفورز شده و با روش نیترات نقره رنگ آمیزی گردیدند. تمامی جایگاه­ها چند­شکل بودند. میانگین تعداد آلل­ها و محتوا­ی اطلاعات چند شکلی (PIC) برای جمعیت آرتمیا فرانسیسکانا و آرتمیا اورمیانا به­ترتیب برابر با 3، 5428/0 و 5/2، 3833/0 بود. در آرتمیا اورمیانا تمامی جایگاه­ها در تعادل هاردی-وینبرگ قرار داشتند، در حالی که در آرتمیا فرانسیسکانا تنها جایگاه Af-A136 در تعادل هاردی-وینبرگ قرار داشت. میانگین هتروزیگوسیتی مورد انتظار برای آرتمیا فرانسیسکانا و آرتمیا اورمیانا به­ترتیب 6209/0 و 4531/0 محاسبه شد. دندروگرام فیلوژنتیکی در داخل جمعیت­ها براساس فاصله­ی ژنتیکی و با استفاده از روش UPGMA ترسیم گردید. براساس تعداد آلل­ها و فراوانی آنها در جمعیت آرتمیا­ها­ی مورد بررسی می­توان دریافت که این جمعیت­ها از ترکیب ژنتیکی مناسبی برخوردار هستند.

کلیدواژه‌ها


عنوان مقاله [English]

Genetic identification and classification of Iranian Artemia using microsatellite markers

نویسندگان [English]

  • Reza Zeynal Pour
  • Seyyed Ziya Aldin Mir Hosseini
  • Seyyed Benyamin Dalir Sefat
  • Jalal Zare
چکیده [English]

In this study genetic variation of Artemia urmiana and Artemia franciscana populations were assessed using five microsatellite markers including Af-B105TAIL, Af-A136, Apdq03TAIL, Apdq04TAIL and Apdq05TAIL from Artemia franciscana and Artemia parthenogenetica. DNA was extracted from 50 cysts of Artemia urmiana and Artemia franciscana populations individually by Hot Shot method. Polymerase chain reactions (PCR) were successfully conducted with all primers and then the PCR products were electrophoresed using 6% none denaturing gel and stained using silver nitrate method. Hence, all alleles were polymorphic. Average number of alleles and polymorphic information content (PIC) for Artemia urmiana and Artemia franciscana populations were 3.0, 0.54 and 2.5, 0.38, respectively. All loci in Artemia urmiana were in HWE but only the Af-A136 locus in Artemia franciscana was in HWE. The average expected heterozygosity for Artemia franciscana and Artemia urmiana were estimated as 0.6209 and 0.4531, respectively. The phylogeny dendrogram based on the Distance Matrix was drawn using UPGMA for within populations. Our findings demonstrated that microsatellite markers could be an appropriate tool for screening biodiversity in animals. Therefore, extinction of these invaluable genetic resources can be preserved using accurate breeding and management programs.

کلیدواژه‌ها [English]

  • microsatellite
  • Artemia
  • genetic diversity
  • Heterozygosity
  • polymorphism
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