ارزیابی مقایسه‌ای نشانگرهای CBDP و SCoTدر بررسی تنوع ژنتیکی موجود در توده‌های مختلف Aegilops

نوع مقاله : مقاله پژوهشی

نویسندگان

1 موسسه تحقیقات اصلاح و تهیه نهال و بذر، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران

2 کرمانشاه دانشگاه آزاد اسلامی واحد کرمانشاه

3 گروه اصلاح نباتات، دانشکده کشاورزی، واحد کرمانشاه، دانشگاه آزاد اسلامی، کرمانشاه، ایران

4 دانشکده علوم و مهندسی کشاورزی دانشگاه تهران، گروه زراعت و اصلاح نباتات، دانشگاه تهران، ایران

چکیده

هدف: اهداف اصلی این مطالعه ارزیابی تنوع ژنتیکی موجود در 103 توده Aegilops و مقایسه کارایی دو نشانگر مولکولی SCoT (Start codon targeted) و CBDP (CAAT box-derived polymorphism) بود.
مواد و روش­ها: در این مطالعه تنوع ژنتیکی 103 توده وحشی متعلق به هفت گونه Aegilops شامل هفت نمونه از Ae. caudata، 14 نمونه از Ae. crassa، 19 نمونه از Ae. cylindrica، 11 نمونه از Ae. neglecta، 20 نمونه از گونه Ae. tauschii، 15 نمونه از Ae. triuncialisو 17 نمونه از Ae. umbellulata استفاده شد با استفاده از 30 آغازگر SCoT و CBDP مورد ارزیابی قرار گرفتند.
نتایج: در مجموع 15 آغازگر SCoT و 15  آغازگر CBDP به ترتیب 164 و 141 قطعه چند شکل تکثیر کردند. متوسط کلیه شاخص­های تعیین کننده کارایی نشانگرهای مولکولی برای آغازگرهای SCoT بیشتر از CBDP بود. هر دو سیستم نشانگری از مقدار PIC یکسانی برخوردار بودند. نتایج تجزیه واریانس مولکولی (AMOVA) نشان داد بیشترین سهم واریانس ژنتیکی مربوط به درون گونه­ها می­باشد. مقایسه پارامترهای ژنتیکی درون جمعیتی نشان داد که در بین گونه­های مورد ارزیابی، Ae. cylindricaنسبت به سایر گونه­ها از تنوع بیشتری برخوردار بود. تجزیه خوشه­ای بر اساس هر یک از سیستم­های نشانگری کلیه توده­های مورد بررسی را به دو گروه اصلی تفکیک نمود. الگوی گروه­بندی به وجود آمده بر اساس آغازگرهای CBDP روند فیلوژنتیکی مشخصی را بین برخی از گونه­های Aegilops نشان داد، به­طوریکه نتایج تجزیه به مختصات اصلی (Principal Coordinates Analysis) گروه­بندی به دست آمده را تأیید نمود.
نتیجه­گیری: هر دو سیستم نشانگری به خوبی قادر به شناسایی چندشکلی موجود در نمونه­های مورد بررسی بودند، با این حال داده های CBDP الگوی گروه­بندی دقیق­تری را بر اساس روابط فیلوژنتیکی نشان داد. از اینرو استفاده از این نشانگرها به صورت مجزا و یا در ترکیب با سایر نشانگرها در بررسی روابط فیلوژنتیکی توصیه می­شود.

کلیدواژه‌ها


عنوان مقاله [English]

Comparative assessment of SCoT and CBDP markers for investigation of genetic diversity existing in different aegilops species

نویسندگان [English]

  • Alireza Pour-Aboughadareh 1
  • Alireza Etminan 2
  • Lia Shooshtari 3
  • Neda Maleki-Tabrizi 4
1 Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
2 Assistant Professor, Department of Plant Breeding, Kermanshah Brunch, Islamic Azad University, Kermanshah, Iran
3 Department of Plant Breeding, Kermanshah Brunch, Islamic Azad University, Kermanshah, Iran
4 Department of Agronomy and Plant Breeding, Faculty of Agricultural Science and Engineering, University of Tehran, Tehran, Iran
چکیده [English]

Objective
The main goals of this study were investigation of genetic diversity in 103 Aegilops accessions and comparison of the efficiency of start codon targeted (SCoT) and CAAT box-derived polymorphism (CBDP) markers.
 
          Materials and methods
In this study, the genetic diversity in 103 Aegilops accessions belonging to seven species including seven samples of Ae. caudata, 14 samples of Ae. crassa, 19 samples of Ae. cylindrica, 11 samples of Ae. neglecta, 20 samples of Ae. tauschii, 15 samples of Ae. triuncialis and 17 samples of Ae. umbellulata was evaluated using 15 SCoT and 15 CBDP primers.
 
Results
In total, 15 SCoT and 15 CBDP primers amplified 164 and 141 polymorphic bands, respectively. SCoT primers showed the highest values for all of the informativeness parameters than CBDP primers. However, both molecular markers indicated the same PIC values. The results of analysis of molecular variance (AMOVA) revealed that the highest proportion of genetic variance referred to within species. Among all species, Ae. cylindrica had the highest values of genetic parameters. Although cluster analysis based on each marker system classified all accessions into two main groups, the grouping pattern obtained from CBDP data indicated a clear phylogenetic relationship among Aegilops species compared to SCoT data. Besides, the results of clustering were confirmed by principal coordinates analysis (PCoA) analysis.
 
Conclusion
On the whole, both molecular markers revealed good capability in depicting of polymorphism among tested accessions. However, CBDP markers provided a vivid grouping pattern for evaluated samples. Hence, the use of this technique individually or in combination with other molecular markers is recommended for phylogenetic assessments. 

کلیدواژه‌ها [English]

  • Aegilops
  • genetic diversity
  • molecular targeted-markers
  • principal coordinates analysis
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