ارتباط نشانگری و تنوع ژنتیکی صفات زراعی گلرنگ (Carthamus tinctorius L.) با استفاده از نشانگر AFLP

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش آموخته کارشناسی ارشد، دانشکده کشاورزی، دانشگاه شهید باهنر کرمان

2 استادیار/بهنزادی گیاهی پژوهشکده فناوری تولیدات گیاهی/ دانشگاه باهنر کرمان

3 دانشیار اصلاح نباتات، قطب علمی تنش‌های محیطی در غلات، دانشکده کشاورزی دانشگاه شهید باهنر کرمان

چکیده

هدف: این آزمایش با هدف بررسی تنوع ژنتیکی، تعیین بهترین ساختار ژنتیکی و تجزیه ارتباطی گلرنگ با استفاده از نشانگر AFLP جهت شناسایی نشانگرهای پیوسته با صفات زراعی مختلف انجام شد.
مواد و روش‌ها: در این مطالعه 17 ژنوتیپ‌ گلرنگ به صورت طرح بلوک کامل تصادفی با 3 تکرار در مزرعه تحقیقاتی دانشگاه شهید باهنر کرمان در سال 1395 کشت گردیدند. صفات فنوتیپی شامل عملکرد دانه، ارتفاع بوته، تعداد قوزه در بوته، تعداد دانه در قوزه، وزن هزار دانه، قطر قوزه، روز تا 50% گلدهی و روز تا رسیدگی اندازه­گیری شد. تکنیک AFLPبا استفاده از هشت ترکیب آغازگری EcoRI و MseI انجام شد.
نتایج: در کل 147 باند چند شکل با میانگین 58/81 درصد چندشکلی ایجاد شد. تجزیه کلاستر بر اساس روش الگوبندی UPGMA و معیار جاکارد ژنوتیپ‌های گلرنگ را به دو گروه تقسیم کرد. تعداد 45 و 39 نشانگر به ترتیب بر اساس مدل GLM و MLM ارتباط معنی‌دار با صفات مورد مطالعه داشتند. نشانگرهای M14/E6-10، M14/E11-16، M14/E11-13، M3/E10-14 و M4/E36-12 با عملکرد دانه، M3/E10-12، M3/E36-29، M59/E36-21 و M14/E11-10 با تعداد قوزه در بوته، M4/E36-8، M59/E36-21، M3/E10-9، M14/E11-14 و M14/E11-13 با تعداد دانه در قوزه، M4/E36-19،12  M4/E36-، M14/E11-1 و M4/E10-1 با وزن هزار دانه، M4/E10-2، M59/E36-21، M3/E36-30  و M4/E36-24 با ارتفاع گیاه، M3/E36-24، M3/E36-6، M3/E10-20 و M4/E36-18  با قطر قوزه، M14/E11-10، M3/E36-30 و M59/E36-21 با روز تا 50% گلدهی و M14/E11-10 و M59/E36-21 با روز تا رسیدگی در هر دو مدل همبستگی معنی‌دار نشان دادند.
نتیجه‌گیری: نشانگرهای AFLP مشخص شده با اثرات قوی در این مطالعه می‌تواند کاندیدهای مناسبی برای انتخاب به کمک نشانگر در برنامه‌های اصلاحی و تبدیل به نشانگرهای اختصاصی دیگر باشند.

کلیدواژه‌ها

عنوان مقاله [English]

Marker association and genetic variation of agronomic traits in safflower )Carthamus tinctorius L.( using AFLP marker

نویسندگان [English]

  • Lida Soltani 1
  • Fatemeh Ebrahimi 2
  • Qasem Mohammadi Nezhad 3
1 . M. Sc. Graduate, College of Agriculture, Shahid Bahonar University of Kerman
2 Assistant Professor, Research & Technology Institute of Plant Production, Shahid Bahonar University of Kerman, Iran.
3 Associate Professor, College of Agriculture and Research & Technology Institute of Plant Production, Shahid Bahonar University of Kerman, Iran
چکیده [English]

Objective
This experiment was conducted in order to assess genetic variation, determine of the best genetic structure and association analysis of safflower to identify markers associated with different agronomic traits.
 
          Materials and methods
In this study, 17 genotypes of safflower were planted as the randomised complete block design at research farm of Shahid Bahonar University of Kerman in 2016. Traits including seed yield, plant height, number of capitulum per plant, number of seed per capitulum, 1000- seed weight, capitulum diameter, day to 50% flowering and day to maturity were measured. The AFLP Technique was performed by the eight EcoR1/Mse1 primer combinations.
          Results
In total, 147 polymorphic bands were generated with average 81.58 polymorphic percentage. Cluster analysis using UPGMA and Jacard as similarity index discriminated safflower genotypes into two groups. Based on GLM and MLM model, 45 and 39 markers had significant association with the studied traits, respectively. M14/E6-10, M14/E11-16, M14/E11-13, M3/E10-14 and M4/E36-12 markers with seed yield, M3/E10-12, M3/E36-29, M59/E36-21 and M14/E11-10 with capitulum number per plant, M4/E36-8, M59/E36-21, M3/E10-9, M14/E11-14 and M14/E11-13 with number of seed per capitulum, M4/E36-19, M4/E36-12, M14/E11-1 and M4/E10-1 with 1000- seed weight, M4/E10-2, M59/E36-21, M3/E36-30, M4/E10-11 and M4/E36-24 with plant height, M3/E36-24, M3/E36-6, M3/E10-20 and M4/E36-18 with capitulum diameter, M14/E11-10, M3/E36-30 and M59/E36-21 with day to 50% flowering, M14/E11-10 and M59/E36-22 markers with day to maturity in both models.
 
         Conclusions
Detected AFLP markers with strong effects in this study could be desirable                                                                                                                                              candidates for the marker-assisted selection in breeding programs and conversion into other specific markers.

کلیدواژه‌ها [English]

  • Association analysis
  • marker coefficient of determination
  • cluster analysis
  • mixed linear model

مراجع

موسوی دراز محله مهسا؛ زین ‌العابدینی مهرشاد؛ مردی محسن؛ مرعشی حسن؛ ملک‌ زاده سعید؛ کاظمی مهربانو؛ رودبار شجاعی طه؛ زهراوی مهدی (1392) بررسی تنوع ژنتیکی و تجزیه ساختار جمعیت ژرم‌پلاسم انار شیرین ایران با استفاده از نشانگر‌های SSR. مجله بیوتکنولوژی کشاورزی 4 (5)، 138- 150.
نقوی محمد رضا؛ قره یاضی بهزاد؛ حسینی سالکده قاسم (1387) نشانگرهای مولکولی. چاپ و انتشارات دانشگاه تهران، 109-121.
 
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مجله بیوتکنولوژی کشاورزی
دوره 12، شماره 2
شهریور 1399
صفحه 43-62

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