بازسازی شبکه بیوسنتزی منولیگنول‌ها و بررسی نقش ژن‌های سیناموئیل کوآ (CsCCR) در مراحل مختلف نموی دانه روغنی کاملینا (Camelina sativa)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه زیست فناوری کشاورزی،پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری، تهران، ایران

2 گروه زیست شناسی، دانشکده علوم، دانشگاه سیستان و بلوچستان، زاهدان، ایران

3 پژوهشگاه ملی مهندسی ژنتیک

چکیده

هدف: امروزه کشت کاملینا به دلیل بروز بحران‌های ناشی از مصرف انرژی‌های فسیلی، علاوه بر مصارف خوراکی، با هدف تولید سوخت زیستی هم مورد توجه قرار گرفته‌است. پژوهش حاضر ژن‌های درگیر در بیوسنتز منولیگنول‌ها را به لحاظ بیان در مراحل نموی مختلف در دانه روغنی کاملینا بررسی نموده‌است. منولیگنول‌ها جریان کربن را یا به سمت تقویت جداره سلول با افزایش لیگنینی شدن می‌برند و یا به سوی تولید ترکیبات شیمیایی مثل کملکسین هدایت می‌کنند. هر دو خصوصیت در اعطای مقاومت گیاه به بیماری‌ها اهمیت دارند.
مواد و روش‌ها: در این مطالعه از داده‌های TPM مربوط به ژن‌های کارکردی و عوامل رونویسی درگیر در بیوسنتز منولیگنول در گیاه کاملینا استفاده شد. بیان این ژن‌ها در مراحل نرمال نموی مختلف دچار تغییر می‌شوند. با استفاده از زبان برنامه نویسی R این داده‌ها برای ساده‌سازی نمایش نتایج، تحلیل‌گردیدند.
نتایج: ژن‌های CsCCR1 و CsCCR4 به خوبی در بافت‌های مختلف بیان شدند درحالیکه ژن CsCCR2 به میزان بسیار ناچیزی در شرایط نرمال بیان می‌شود. در عوض مدارک دیگر نشان دادند که ژن اخیر و ژن CsCCR4 تحت القای قارچ Sclerotinia sclerotiorum در کاملینا افزایش چشمگیری در بیان نشان می‌دهد. مقایسه شرایط نرمال رشد و مواقع حمله پاتوژن نشان داد که ژن CsCCR4بطور مداوم درحال بیان است و گویای اینست که این ژن همزمان دارای نقش ساختاری و دفاعی می‌‌باشد. این دو ژن‌ اخیر می‌توانند به عنوان منبع بالقوه مقاومت به بیماری مورد بررسی‌های بیشتر قرار گیرند. بطور خودکار، شبکه‌های تنظیمی بیان ژن از جمله کلیدهای اصلی نموی و عوامل رونویسی در کاملینا در برقراری تعادل میان دوگانه رشد طبیعی و سازوبرگ‌های دفاعی حاصل از مسیر بیوسنتز منولیگنول بسته به نوع پیام‌های دریافتی، عمل می‌کنند هرچند که پاسخ آفریده شده وابسته به جوهره و هویت رقم می‌باشد .

کلیدواژه‌ها


عنوان مقاله [English]

Toward evaluation of the monolignol biosynthesis gene network with contemplation on the role of cinnamoyl coA reductase (CCR) gene family in camelina sativa

نویسندگان [English]

  • Naser Darvishi 1
  • Morteza Sabri 2
  • Mehdi Alavi 3
1 Department of Agricultural Biotechnology, National Institute of Genetic Engineering and Biotechnology,Tehran, Iran.
2 Department of Biology, Faculty of Science, University of Sistan va Baluchestan, Zahedan, Iran.
3 Associate Professor, Department of Agricultural Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
چکیده [English]

 
Objective
Camelina (Camelina sativa L. Crantz) is a fast-growing oil crop belonging to the Brassicaceae family that can tolerate drought, salinity, cold, and many diseases and pests. Camelina seed has precious oil and protein with a number of potential attributes or benefits in both the human food and animal feed industry. Camelina also is being deemed as promising species to produce biodiesel and jet fuel in margin lands of the globe. Monolignols, as the precursor of lignin, are the common compounds in both lignification and soluble chemicals that have important roles in both normal development of healthy plants and defense-related responses in infected plant subjects. The regulatory mechanisms underlying the biosynthesis of these multifaceted secondary metabolites are poorly understood.
 
 
Material and Methods
Our current study presents the mode of gene expression and analyzes data to investigate the role of monolignol biosynthesis genes in the normal development growth of Camelina. We considered the transcript level of those genes that were covered 12 different tissues in major developmental stages during the life cycle of the Camelina. Using the R programming environment, we could have visualized the pattern of gene expressions with transcript per million (TPM) data in the heatmap. 
 
Results
The results revealed the similarities as well as differences in gene expression patterns in both regulatory and functional gene groups among different tissues. Moreover, tissue-specific genes in different developmental stages were recognized. 
 
Conclusions
The scrutiny in the literature related on biotic stress experiments in Camelina and also other species determined considerable differences in transcript levels and gene regulation patterns for the genes especially for members of the gene group encoding cinnamoyl-CoA reductase (CCR). Association of the latter genes CsCCR4 and CsCCR2 in particular involved in monolignol biosynthesis with the resistance of Camelina to pathogens contributes to providing a preliminary view to contemplate the future research options in various Camelina breeding programs.

کلیدواژه‌ها [English]

  • Biofuel
  • Camelina oilseed crop
  • Cinnamoyl CoA reductase
  • Monolignol Biosynthesis
 
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