بیان ژن HSP90 و ارتباط آن با دمای محیط و تردد زنبور عسل ایرانی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه علوم دامی، دانشکده کشاورزی و منابع طبیعی، دانشگاه خلیج فارس، بوشهر، ایران

2 گروه گیاه پزشکی، دانشکده کشاورزی و منابع طبیعی، دانشگاه خلیج فارس، بوشهر، ایران

چکیده

هدف: پروتئین HSP90 یکی از اعضای خانواده پروتئین‌های شوک گرمایی است که در پاسخ به استرس‌های محیطی تولید شده و نقش‌های مختلفی در سلول دارد. در این مطالعه تغییر بیان ژن HSP90 در ساعات مختلف روز و رابطه آن با دمای محیط و تردد زنبور عسل کارگر مورد بررسی قرار گرفت.
مواد و روش: تردد زنبور عسل چراگر در جلوی دریچه کندو و دمای محیط در پنج زمان مختلف روز (ساعت‌های 7:00، 9:30، 12:00، 14:30 و 17:00) طی سه روز اندازه‌گیری شد. بیان نسبی ژن HSP90 نیز طی این پنج زمان با استفاده از روش Real-time PCR اندازه‌گیری شد. برای آنالیز آماری داده‌های دما و تردد زنبور عسل، اثر روز به عنوان بلوک (اثر تصادفی و هر روز به عنوان یک بلوک) و اثر زمان‌های مختلف روز به عنوان اثر ثابت در مدل مد نظر قرار داده شد. همچنین تابعیت بین این صفات نیز مورد بررسی قرار گرفت.
نتایج: نتایج نشان داد که ساعت‌های مختلف روز اثر معنی‌داری بر تردد زنبور عسل و بیان نسبی ژن HSP90 دارد (01/0 > P). حداکثر و حداقل دمای محیط به ترتیب در ساعت‌های 12:00 ظهر و 7:00 صبح ثبت شد. حداکثر و حداقل تردد زنبورهای چراگر نیز به ترتیب در ساعت‌های 17:00 و 12:00 ظهر حاصل شد. به علاوه اینکه بیشترین و کمترین میزان بیان نسبی ژن HSP90 در ساعت‌های 12:00 و و7:00 ملاحظه شد. نتایج آنالیز تابعیت مشخص کرد که تردد زنبور کارگر و بیان نسبی ژن HSP90 با افزایش دمای محیط افزایش می‌یابد؛ البته تردد زنبورها در دمای بالاتر از ۳۹ درجه سلسیوس کاهش یافت. به علاوه، تردد زنبورهای کارگر تا افزایش سه برابری بیان ژن HSP90، افزایشی بود و بعد از آن روند کاهشی داشت.
نتیجه‌گیری: بطور کلی روند تردد زنبور عسل، دمای محیط و بیان نسبی ژن HSP90 طی روز الگوی مشخصی داشت. افزایش دما و وارد شدن یک تنش دمایی در اواسط روز باعث افزایش بیان ژن HSP90 و در نتیجه کاهش تردد زنبور عسل شد. توصیه می‌شود به منظور بهینه‌سازی تردد زنبورهای عسل جهت جمع آوری شهد، مدیریت لازم جهت کنترل این تنش دمایی اعمال شود. 

کلیدواژه‌ها


عنوان مقاله [English]

Expression of hSP90 gene and its relationship with ambient temperature and foraging rate in apis mellifera meda

نویسندگان [English]

  • Salem Morammazi 1
  • Mohammad Alii Mirhosseini 2
1 Department of Animal Science, Faculty of Agricultural and Natural Resources, Persian Gulf ,University, Bushehr, Iran
2 Department of Plant Protection, Faculty of Agricultural and Natural Resources, Persian Gulf University, Bushehr, Iran.
چکیده [English]

Objective
HSP90 is a member of heat shock proteins (HSPs) family which produces in response to environmental stress factors and has different roles in cells. In this study, the differential expression of HSP90 gene during the day and its relationship with ambient temperature and foraging rate of worker bees were investigated.
 
Material and methods
The worker bees’ commuting in front of hive and ambient temperature in five different times of day (at 7:00, 9:30, 12:00, 14:30 and 17:00) were recorded for three days. The relative transcription level of HSP90 gene was measured in those times using real-time PCR method. The effects of day and different times in a day were subjected to the model as random and fixed effects, respectively, for analyzing worker bees’ commuting and ambient temperature. The regression between these traits was also run.
 
Results
Results showed that time of day has significant effect on ambient temperature, worker bees’ commuting and relative expression of HSP90 (P < 0.01). The highest and lowest ambient temperature were recorded at 12:00 pm and 7:00 am, respectively. The highest and least worker bees’ commuting were obtained at 17:00 and 12:00 pm, respectively. In addition, the highest and lowest HSP90 gene transcription level were recorded at 12:00 pm and 7:00 am o’clock, respectively. The regression analysis revealed that worker bees’ commuting and relative expression of HSP90 gene were increased with increasing of ambient temperature; the commuting of bees was reduced in temperature above 39 ̊ C. Furthermore, the worker bees’ commuting was increased with increasing of HSP90 expression to three-fold, which was decreased with more relative expression level.
 
Conclusion
In general, the trend of worker bees’ commuting, ambient temperature, and relative expression of HSP90 gene were clear during the day. When the ambient temperature was increased, the relative transcription level of HSP90 gene was increased in response to heat stress, while the number of commuter bees was decreased. The management of heat shock can be recommended to improve nectar gathering by commuter bees.
 
 

کلیدواژه‌ها [English]

  • HSP90 Gene
  • Gene expression
  • Foraging Behavior
  • Worker Bee
  • Ambient Temperature
  • Apis mellifera meda
 
بهادر یاسر ، محمدآبادی محمدرضا ، خضری امین ، اسدی مهدیه ، مدحتی لیلا (1395) مطالعه تنوع ژنتیکی جمعیتهای زنبور عسل استان کرمان با استفاده از نشانگرهای  ISSR. پژوهش‌های تولیدات دامی 13، 192-186.
توحیدی نژاد فاطمه، محمدآبادی محمدرضا، اسمعیلی زاده کشکوئیه علی، نجمی نوری عذرا (1393) مقایسه سطوح مختلف بیان ژنRheb  در بافت‌های مختلف بز کرکی راینی. مجله بیوتکنولوژی کشاورزی 6، 50-35. 
محمدآبادی محمدرضا (1398) بیان ژن کالپاستاتین در بز کرکی راینی با استفاده از Real Time PCR. مجله بیوتکنولوژی کشاورزی 11، 235-219. 
محمودی مریم ، آیت اللهی احمد ، محمدآبادی محمدرضا (1396). بررسی اگزون چهارم ژن کاپاکازئین گوسفند کرمانی با تکنیک PCR-RFLP. مجله بیوتکنولوژی کشاورزی 9، 119-128.
مرمضی سالم، مسعودی علی اکبر، واعظ ترشیزی رسول، پاکدل عباس (1393). تمایز بیان ژن انتقال دهنده نوع 1 گلوکز (GLUT1) در زمان‌های متفاوت فیزیولوژیکی در غدد پستانی بزهای عدنی ایران. مجله بیوتکنولوژی کشاورزی 6، 159-173.
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