ساخت واکسن پلی اپی توپی بر علیه بیماری نیوکاسل بوسیله طراحی ایمونوانفورماتیکی پروتئین های ایمنی زا

نوع مقاله : مقاله پژوهشی

نویسندگان

1 عضو هیات علمی دانشگاه جیرفت

2 شیلات- دانشده علوم دامی و شیلات - دانشگاه کشاورزی و منابع طبیعی ساری

3 بخش علوم دامی دانشکده کشاورزی دانشگاه جیرفت

:10.22103/jab.2021.17424.1309

چکیده

هدف: استفاده از تکنیک‌های زیست‌شناسی مولکولی برای تولید واکسن‌های جدید علیه سویه‌های مختلف ویروس بیماری نیوکاسل (NDV)  موضوع گزارش‌های تحقیقاتی اخیر بوده است. توسعه تکنیک‌های بهبودیافته برای تعیین توالی ژنوم منجر به شناسایی مکانیسم‌های محافظتی و شناسایی آنتی‌ژن‌های احتمالی کاندید شده است؛ تحقیق حاضر با هدف طراحی یک اپی توپ نوترکیب در برابر عوامل اتصال در ویروس نیوکاسل در پرندگان انجام شده است.
مواد و روش‌ها: در این تحقیق برای ساختن واکسن نوترکیب از روش‌های بیوانفورماتیکی استفاده شده است تا با استفاده از آنتی‌ژن‌های ضد این ویروس، آنتی‌ژن‌های HN و F این بیماری را کنترل و از بین ببریم. برای اتصال این اپی توپ‌ها، از لینکرهای انعطاف‌پذیر مانند AAY  و  KK به‌عنوان پیونددهنده‌های ساختار انتخاب شدند. این ساختار حاوی 309 اسیدآمینه است. فاکتورهای مهم بیولوژیکی این واکسن نوترکیب مانند خصوصیات فیزیکی-شیمیایی، ساختارهای مختلف، پایداری، اختلال پروتئین ذاتی، حلالیت و حساسیت‌زایی این ساختار واکسن با استفاده از تجزیه‌وتحلیل سیستم ایمنی بدن ارزیابی شد.
نتایج: تجزیه ‌و تحلیل‌های مختلف پایداری این ساختار را تأیید کرد و اپی توپ‌های پیش‌بینی‌شده در واکسن نوترکیب پتانسیل بالایی را برای القای پاسخ ایمنی سلول Bهای و T نشان داد؛ بنابراین، تجزیه‌وتحلیل سیستم ایمنی نشان داد که واکسن چند اپی توپی می‌تواند به ‌درستی پاسخ‌های ایمنی سلول T و B را تحریک کند و به طور بالقوه می‌تواند برای برنامه‌های پیشگیری یا کنترل استفاده شود. از نتایج این مطالعه می‌توان برای کنترل و از بین بردن بیماری نیوکاسل در آینده پس از تأیید اثربخشی آن با استفاده از روش‌های ایمونولوژیک تجربی استفاده کرد.

کلیدواژه‌ها


عنوان مقاله [English]

Construction of a polyepitope vaccine against Newcastle disease by immunoinformatics design of immunogenic proteins

نویسندگان [English]

  • zahra rodbari 1
  • Abdolvahab Ebrahimpour Gorji 2
  • Arsalan Brazandeh 3
1 Zahra Roudbari, Department of Animal Science, Faculty of Agriculture, University of Jiroft, Jiroft, Iran
2 Sari University of Agricultural Sciences and Natural Resources, Sari, Iran Department of Animal Science and fishery
3 Department of Animal Science, Faculty of Agriculture, University of Jiroft, Jiroft, Iran
چکیده [English]

Objective
Application of molecular biology techniques to the production of new vaccines against different strains of the Newcastle disease virus (NDV) has been the subject of recent research reports. Development of improved techniques for genome sequencing has led to the recognition of protective mechanisms and the identification of possible candidate antigens. The present research aimed to design a recombinant chimeric immunogen against binding agents in Newcastle virus in birds.
 
Materials and methods
Therefore, we conducted this investigation to make a recombinant vaccine in silico in order to control and eliminate this disease using anti-leptospirosis epitopes, F, and HN antigens. To bind these epitopes, KK, as AAY linkers, were selected to bind the structure. This structure contained 309 amino acids. The important biological factors of this recombinant vaccine, such as physicochemical properties, different structures, stability, intrinsic protein disorder, solubility, and allergenicity of this vaccine structure were evaluated using immune system analysis.
 
Results
Various analyses confirmed the stability of this structure, and the epitopes predicted in the chimeric vaccine showed a high potential for inducing the immune response of B cell and T cell. Thus, immune system analysis revealed that the modeled multi-epitope vaccine could properly stimulate T and B cell immune responses and could potentially be utilized for prophylactic or control applications.
 
Conclusion
The results of this study could be employed to control and eliminate leptospirosis in the future after confirming its effectiveness by experimental immunological assays

کلیدواژه‌ها [English]

  • Newcastle
  • Vaccine design
  • F and HN
  • Multi- epitope
 
 
References
Ahsani M, Bafti MS, Esmailizadeh A et al. (2011) Genotyping of isolates of Clostridium perfringens from vaccinated and unvaccinated sheep. Small Rumin Res 95, 65-69.
Alexander DJ, Bell JG, Alders RG (2004) A technology review: Newcastle disease, with special emphasis on its effect on village chickens.
Argos P (1990) An investigation of oligopeptides linking domains in protein tertiary structures and possible candidates for general gene fusion. J Mol Biolog 211, 943-958.
Arnon R, Ben-Yedidia T (2003) Old and new vaccine approaches. Int Immunopharmacol 3, 1195-1204.
Awan MA, Otte M, James A (1994) The epidemiology of Newcastle disease in rural poultry: a review. Avian Pathol 23, 405-423.
Boursnell M, Green P, Samson A et al. (1990) A recombinant fowlpox virus expressing the hemagglutinin-neuraminidase gene of Newcastle disease virus (NDV) protects chickens against challenge NDV. Virol 178, 297-300.
Chen X, Zaro JL, Shen W-C (2013) Fusion protein linkers: property, design and functionality. Adv Drug Deliv Rev 65, 1357-1369.
Collins M, Bashiruddin J, Alexander D (1993) Deduced amino acid sequences at the fusion protein cleavage site of Newcastle disease viruses showing variation in antigenicity and pathogenicity. Arch Virol 128, 363-370.
Colovos C, Yeates TO (1993) Verification of protein structures: patterns of nonbonded atomic interactions. Protein Sci 2, 1511-1519.
Cosset F-l, Bouquet J-F, Drynda A et al. (1991) Newcastle disease virus (NDV) vaccine based on immunization with avian cells expressing the NDV hemagglutinin-beuraminidase glycoprotein. Virol 185, 862-866.
Dimitrov I, Flower DR, Doytchinova I (2013) AllerTOP-a server for in silico prediction of allergens. In: BMC bioinformatics. BioMed Central. pp. 1-9.
Gasteiger E, Hoogland C, Gattiker A et al. (2005) Protein identification and analysis tools on the ExPASy server. The proteomics protocols handbook, 571-607.
Gotoh B, Sakaguchi T, Nishikawa K et al. (1988) Structural features unique to each of the three antigenic sites on the hemagglutinin-neuraminidase protein of Newcastle disease virus. Virol 163, 174-182.
Janson G, Zhang C, Prado MG et al. (2017) PyMod 2.0: improvements in protein sequence-structure analysis and homology modeling within PyMOL. Bioinformatic 33, 444-446.
Le-Barillec K, Magalhaes JG, Corcuff E et al. (2005) Roles for T and NK cells in the innate immune response to Shigella flexneri. J Immunol 175, 1735-1740.
Lee Y-J, Sung H-W, Choi J-G et al. (2008) Protection of chickens from Newcastle disease with a recombinant baculovirus subunit vaccine expressing the fusion and hemagglutinin-neuraminidase proteins.J Vet Sci 9, e301.
Lovell SC, Davis IW, Arendall III WB et al. (2003) Structure validation by Cα geometry: ϕ, ψ and Cβ deviation. Protein 50, 437-450.
María R, Arturo C, Alicia JA et al. (2017) The impact of bioinformatics on vaccine design and development. InTech, Rijeka, Croatia.
Meulemans G, Letellier C, Gonze M et al. (1988) Newcastle disease virus f glycoprotein expressed from a recombinant vaccinia virus vector protects chickens against live‐virus challenge. Avian Pathol 17, 821-827.
Motamedi MJ, Amani J, Shahsavandi S et al. (2014) In silico design of multimeric HN-F antigen as a highly immunogenic peptide vaccine against Newcastle disease virus. Int J Pept Res Ther 20, 179-194.
Nagy E, Krell P, Dulac G et al. (1991) Vaccination against Newcastle disease with a recombinant baculovirus hemagglutinin-neuraminidase subunit vaccine. Avian Dis 15, 585-590.
Naohiro K, Masahiro N, Mitsuru O et al. (1994) Protective effect of individual glycoproteins of Newcastle disease virus expressed in insect cells: the fusion protein derived from an avirulent strain had lower protective efficacy. Virus Res 32, 373-379.
Osman MM, ElAmin EE, Al-Nour MY et al. (2016) In silico design of epitope based peptide vaccine against virulent strains of hn-newcastle disease virus (NDV) in poultry species. IJMCR: Int J Curr Multidiscip Stud 4.
Permin A, Pedersen G, Riise J (2001) Poultry as a tool for poverty alleviation: Opportunities and problems related to poultry production at village level. In: ACIAR proceedings. ACIAR; 1998. pp. 143-147.
Peters B, Sidney J, Bourne P et al. (2005) The design and implementation of the immune epitope database and analysis resource. Immunogenet 57, 326-336.
Russell P (1988) Monoclonal antibodies in research, diagnosis and epizootiology of Newcastle disease. In: Newcastle disease. Springer. pp. 131-146.
Saha S, Raghava G (2006) AlgPred: prediction of allergenic proteins and mapping of IgE epitopes. Nucleic Acids Res 34, W202-W209.
Temizoz B, Kuroda E, Ishii KJ (2016) Vaccine adjuvants as potential cancer immunotherapeutics. Int  Immunol 28, 329-338.
Vajda S, Yueh C, Beglov D et al. (2017) New additions to the C lus P ro server motivated by CAPRI. Protein 85, 435-444.
Wiederstein M, Sippl MJ (2007) ProSA-web: interactive web service for the recognition of errors in three-dimensional structures of proteins. Nucleic Acid Res 35, W407-W410.
Yang J, Zhang Y (2015) I-TASSER server: new development for protein structure and function predictions. Nucleic Acid Res 43, W174-W181.
Zarrabi A, Alipoor Amro Abadi M, Khorasani S et al. (2020) Nanoliposomes and tocosomes as multifunctional nanocarriers for the encapsulation of nutraceutical and dietary molecules. Mol 25, 638.
Zhang J, Chen Y, Qi J et al. (2012) Narrow groove and restricted anchors of MHC class I molecule BF2* 0401 plus peptide transporter restriction can explain disease susceptibility of B4 chickens. J  Immunolog 189, 4478-4487.