الگوی پروتئینی برگ ارقام متحمل و حساس کلزا تحت تنش خشکی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه بهنژادی و بیوتکنولوژی گیاهی، دانشکده کشاورزی، دانشگاه تبریز،

2 دانشگاه تبریز دانشکده کشاورزی گروه بیوتکنولوژی

چکیده

هدف: تنش خشکی یکی از عمده‌ترین محدودیت‌ها در تولید محصولات زراعی است که اثر نامطلوبی بر کمیت و کیفیت آن‌ها دارد. کلزا همانند بسیاری از گیاهان زراعی از تنش ناشی از کمبود آب متاثر می‌شود. در سطح سلولی گیاهان از طریق سنتز پروتئین‌های خاص به تنش خشکی پاسخ می‌دهند. از این رو پژوهشی با هدف مطالعه مکانیسم پاسخ کلزا به تنش خشکی و تعیین پروتئین‌های دخیل در تحمل تنش انجام شد.
مواد و روش‌ها: الگوی پروتئینی برگ ارقام Hyola308 و  Sarigol بترتیب به عنوان ارقام حساس و متحمل با استفاده از تکنیک پروتئومیک فاقد ژل/فاقد (شاتگان پروتئومیکس) برچسب تحت سطوح مختلف تنش خشکی (6/. و 2/1 مگا پاسکال (MPa) به همراه شاهد) مورد بررسی قرار گرفت. جهت اعتبارسنجی تغییر محتوای پروتئین‌های شناسایی شده از وسترن‌بلات استفاده شد.
نتایج: در کل 56 پروتئین شامل 16 پروتئین به صورت اختصاصی برای Hyola308، 16 پروتئین به صورت اختصاصی برای Sarigol و 12 پروتئین مشترک بین هر دو شناسایی شد. در Sarigol فراوانی اکثر پروتئین‌های درگیر در متابولیسم پروتئین، فتوسنتر و انرژی در مواجهه با سطوح مختلف تنش خشکی کاهش نشان داد، در مقابل در Hyola308 افزایش در فراوانی پروتئین‌های درگیر در متابولیسم انرژی، فتوسنتز و دفاع آنتی‌اکسیدان به صورت اختصاصی مشاهده شد.
نتیجه‌گیری: استنباط می‌شود که افزایش فراوانی این پروتئین‌ها در برگ‌های Hyola308 قسمتی از مکانیسم تحمل این رقم در مواجهه با تنش می‌باشد و تقلیل کارایی چرخه کلوین و کاهش تولید قند و انرژی در Sarigol، کاهش رشد این رقم را تحت تنش خشکی می‌تواند توجیه کند.

کلیدواژه‌ها


عنوان مقاله [English]

Leaf protein pattern of tolerant and susceptible canola cultivars under drought stress

نویسندگان [English]

  • Bita Kazemi Oskuei 1
  • Ali Bandehagh 2
1 Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
2 Breeding for Abiotic Stresses Lab., Dept. of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz 5166616471, Iran.
چکیده [English]

Objective
Drought stress is the main restriction factor in crop production that has an adverse effect on crop quantity and quality. Canola, like many crops, is affected by stress due to water deficit. At the cellular level, plants respond to drought stress by synthesizing specific proteins. Therefore, a research with the aim of studying the response mechanism of canola to drought stress and determining proteins involved in mediating stress tolerance was carried out.
 
Materials and methods
In order to comprehend a mechanism of canola plant response to drought stress, the protein profiles of the drought-tolerant Hyola308 and drought-sensitive Sarigol leaf under different drought stress conditions based on a gel-free/label-free proteomic technique were investigated. To validate the content variation of proteins identified in the proteomic analysis, Western blot analysis was used.
 
Results
A total of 56 proteins were identified in Sarigol and Hyola308, 16 proteins were specific to Hyola308 and 16 proteins were specific to Sarigol, respectively. Of the identified proteins, 12 proteins were commonly detected between Sarigol and Hyola308. In Sarigol under different drought stress conditions, the abundance of proteins related to protein metabolism, photosynthesis and energy metabolism decreased; whereas, in Hyola308, an enhancement in proteins abundance involved in photosynthesis, energy metabolism and antioxidant defense was observed.
 
Conclusions
It is inferred that enhancement of these protein abundance in Hyola308 leaf may be a part of tolerance mechanism of this cultivar exposed to stress and decrease in the Kelvin cycle efficiency and production of sugar and energy in Sarigol may justify growth reduction of this cultivar.

کلیدواژه‌ها [English]

  • abiotic stress
  • Canola
  • Immuno-blot
  • proteomic
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