مکان¬یابی ژنی صفات مرتبط با تحمل به شوری در مراحل زایشی و رویشی برنج

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد بخش بیوتکنولوژی ، دانشکده کشاورزی، دانشگاه شهید باهنر کرمان

2 استادیار بخش زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه شهید باهنر کرمان

چکیده

به منظور مکان­یابی ژن­های صفات کنترل کننده تحمل به شوری در مراحل رویشی و زایشی برنج و تعیین سهم هر مکان ژنی در تنوع فنوتیپی، 80 فرد از جمعیت رگه­های درون زاد نوترکیب حاصل از تلاقیIR29/Pokkali مورد مطالعه قرار گرفتند. امتیاز تحمل ژنوتیپ­ها به شوری، غلظت سدیم، پتاسیم و نسبت سدیم به پتاسیم در مرحله گیاهچه­ای و صفات ارتفاع گیاه، تعداد و وزن دانه، تعداد پانیکول، تعداد پنجه، باروری و عملکرد بیولوژیک در مرحله زایشی اندازه گیری شدند. با استفاده از 81  نشانگر چند شکل SSR، یک   QTLبزرگ اثر برای صفت امتیاز تحمل به شوری در بازوی مقابل Saltol بر روی کروموزوم 1 مشاهده شد، همچنین برای صفات غلظت سدیم، غلظت پتاسیم و نسبت آن­ها، ناحیه بزرگ اثری بر روی کروموزوم­های 1 و 10 مکان یابی شدند که مجموعاً تحمل به شوری در مرحله گیاهچه‎ای را کنترل می نمودند. برای تحمل به شوری در مرحله زایشی چندین QTL بزرگ اثر برای درصد کاهش وزن دانه­های پر و عملکرد بیولوژیک بر روی کروموزوم­های 7 ،9 و 10 مشاهده شد که QTL­های واقع بر روی کروموزوم 10، در مراحل رویشی و زایشی مشترک بود، که می­توان آن را به عنوان ناحیه کاندید جهت نقشه­یابی دقیق و اصلاح به کمک نشانگر مطرح نمود. بنابراین با هرم سازی این ناحیه به همراه Saltol کروموزوم یک می‎توان افراد متحمل به شوری ایجاد نمود.

کلیدواژه‌ها


عنوان مقاله [English]

Gene Mapping of Attributed Traits to Salinity Tolerance at Seedling and Reproductive Stages in Rice

نویسندگان [English]

  • Marjan GHasemkhani 1
  • GHasem Mohammadinejad 2
1
2
چکیده [English]

In order to identify the responsible QTLs attributed to salinity tolerance at seedling and reproductively stages in rice and detection of the ratio of each QTL in phenotypic variation, eighty RILs derived from IR29/Pokkali cross were used. The Sodium (Na) and Potassium (K) concentration, Na/K ratio at seedling stage and height, number and grain weight, number of panicle, number of tillers, fertility and biomass of rice in salinity stress at reproductive stage were assessed as phenotypic traits. Using 81 polymorphic SSR markers, a major QTL related to seedling stage tolerance was detected on the opposite arm of Saltol QTL in chromosome 1. Moreover, a major effected QTL for Na, K and their ratio was found on chromosomes 1 and 10 which totally controlled the tolerance at seedling stage. For tolerance to salinity at reproductive stage; major QTLs detected on chromosome 7, 9 and 10 for Reduction of Filled Grain Weight and Reduction of Biomass Weight . Multi trait QTL which were located on chromosome 10 had the same location with the QTL mapped for seedling stage tolerance, so it can be a good candidate for marker assisted breeding. It can be expected to achieve to the high tolerant genotypes through QTL pyramiding of saltol and chromosomal region of 10 together.

کلیدواژه‌ها [English]

  • QTL mapping
  • Recombination inbred lines (RILs)
  • rice
  • Salinity tolerance

QTL mapping, Recombination inbred lines (RILs), Rice, Salinity tolerance

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